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在非小细胞肺癌中进行液体活检的潜在补充瘤标志物

Jin Xiang¹, Junyan Peng², Zhifang Xing¹

¹Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Experimental biology and medicine (Maywood, N.J.)

|June 13, 2025

概括

这项研究表明,血清数字PCR可以检测非小细胞肺癌 (NSCLC) 患者的EGFR突变,帮助治疗决策. 瘤标志物升高可能有助于预测液体活检中的突变检测.

科学领域:

在瘤学瘤学.
分子诊断学分子诊断学
遗传学是一个遗传学.

背景情况:

精确识别表皮生长因子受体 (EGFR) 氨酸激酶 (TK) 域突变对于非小细胞肺癌 (NSCLC) 治疗和监测对EGFR-氨酸激酶抑制剂 (TKI) 的耐药性至关重要.
液体活检为无法接受传统活检的患者提供了较少侵入性的组织采样替代方案.

研究的目的:

评估基于芯片的新型数字PCR (dPCR) 平台的临床实用性,用于检测NSCLC患者血清中的EGFR外基因19缺失 (19del) 和T790M突变.
探索血清生物标志物用于预测NSCLC中的EGFR突变状态.

主要方法:

收集了350个NSCLC患者的血清样本.
使用dPCR提取无细胞DNA (cfDNA) 并对EGFR突变进行分析 (19del,T790M).
量化了血清瘤标志物水平 (CA199,ProGRP,CA125,CEA) 并将它们与突变检测率相关联.

主要成果:

该dPCR测定显示了19del的73.33%和T790M的28.57%的积极预测值.
针对CA199 (11.75 U/mL) 和ProGRP (45.15 pg/mL) 的特定切割值分别用于19del和T790M的检测.
血清瘤标志物 (CA125,CEA,ProGRP) 的升高与EGFR突变变异型率在阳性队列中呈正相关性.

结论:

基于血清的dPCR液体活检是NSCLC基因定型的潜在有价值的补充工具,补充组织活检.
血清瘤标志物水平可能有助于识别通过液体活检从分子分析中受益的患者.

关键词:

在 EGFR 基因突变.数字PCR是数字的PCR.液体活检活检液体活检非小细胞肺癌是非小细胞肺癌.瘤标志物瘤标志物

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