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使用奇托桑纳米颗粒介导的dsrna传递系统沉默状菌根真菌基因

Chumei Yan¹, Yuemin Wang¹, Qianhuang Guo¹

¹State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China.

|June 13, 2025

概括

研究人员开发了奇托/dsRNA纳米粒子,将双链RNA (dsRNA) 输送到状菌根 (AM) 菌中. 这种方法使得AM真菌的有效基因沉默 (如Rhizophagus irregularis) 能够用于功能研究.

科学领域:

菌类学菌类学是指菌类学.
植物病理学植物病理学
生物技术是生物技术.

背景情况:

植物病原菌可以使用外源双链RNA (dsRNA) 来控制基因沉默.
状菌根 (AM) 菌可以吸收裸体dsrna,但通过纳米载体的传递尚未被探索.

研究的目的:

开发一种有效的方法,使用纳米载体将dsRNA输送到AM真菌中.
为了研究素/dsRNA多复合纳米颗粒 (PNs) 在Rhizophagus irregularis.中对基因沉默的潜力.

主要方法:

在体外合成基托 (CS) /dsRNA多复合纳米颗粒 (PNs).
在Rhizophagus irregularis.中用于基因沉默的CS/dsRNAPN的应用.
使用喷雾诱导基因沉默 (SIGS) 方法进行AM真菌基因表征.

主要成果:

素有效地携带dSRNA,其来源于Rhizophagus不规则的.
在R. irregularis.中,CS/dsRNA PNs成功实现了目标基因沉默.
开发的协议适用于AM真菌的非共生和共生培养.

结论:

建立了一种简单,高效和环保的方法来合成CS/dsRNA PNs.
CS/dsRNA PNs为阐明AM真菌中的基因功能提供了一个有前途的工具.
这种方法促进了AM真菌的基因沉默,用于农业和研究应用.

关键词:

树状菌根菌的真菌树状菌根菌基托桑纳米颗粒基托桑纳米粒子基托桑/dsRNAs 基托桑/dsRNAs 基托桑/dsRNAs 基托桑管不规则是一种不规则的管.喷雾诱导的基因沉默是一种喷雾诱导的基因沉默.dsRNAsRNAs是什么意思

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