一个与分子输送带相关的蛋白质控制了细菌9型分泌系统的旋转方向
Abhishek Trivedi1,2,3, Jacob A Miratsky2,4, Emma C Henderson1,2,3
1School of Life Sciences, Arizona State University, Tempe, Arizona, USA.
细菌中的9型分泌系统 (T9SS) 控制着滑动的运动性. 删除GldJ蛋白的一部分会逆转T9SS的旋转,作为细菌运动的分子开关.
科学领域:
- 细菌细胞生物学 细菌细胞生物学
- 分子微生物学分子微生物学
- 生物物理学的生物物理.
背景情况:
- 9型分泌系统 (T9SS) 对于细菌的滑动性,表面殖民和病变发生至关重要.
- T9SS作为一个旋转电机,通过粘合剂涂层的输送带驱动细菌,类似于分子雪车.
研究的目的:
- 阐明控制T9SS.旋转方向的机制.
- 研究传送带相关蛋白GldJ在T9SS定向性中的作用.
主要方法:
- 在Flavobacterium johnsoniae*中对GldJ蛋白进行基因操纵.
- 对T9SS旋转方向的分析 (CCW与CW).
- 分子动力学 (MD) 模拟T9SS定位器单元 (GldLM).
主要成果:
- 删除GldJ的C终端反转了T9SS从反时钟方向 (CCW) 到时钟方向 (CW) 的旋转.
- GldJ-GldK接口对于控制T9SS方向性至关重要,可能通过调节与GldLM定位器综合体的相互作用.
- 提出了一个"三组件轮"模型,其中GldJ作为T9SS旋转的调节开关.
结论:
- GldJ的C端区域充当分子开关,反转T9SS的旋转方向.
- 包括GldJ,GldK和GldLM在内的T9SS机器作为可控制的生物输送带,允许细菌适应运动.
- 这提供了细菌如何微调其灵活性以响应环境线索的见解.
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