Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cell Culture01:21

Cell Culture

16.9K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
16.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

mSphere of Influence: How a gut microbiota study reshaped my thinking on tick-borne pathogens.

mSphere·2026
Same author

Case Report: Coexistence of a giant borderline phyllodes tumor of the breast and a contralateral fibroadenoma: a challenging case with clinical manifestations and PET-CT imaging mimicking advanced cancer.

Frontiers in oncology·2026
Same author

Correction: S-9-PAHSA ameliorates cognitive decline in a type 2 diabetes mouse model by inhibiting oxidative stress and apoptosis via CAIII modulation.

Frontiers in molecular neuroscience·2025
Same author

S-9-PAHSA ameliorates cognitive decline in a type 2 diabetes mouse model by inhibiting oxidative stress and apoptosis via CAIII modulation.

Frontiers in molecular neuroscience·2025
Same author

<i>Rickettsia parkeri</i> hypothetical protein RPATATE_1266, a homolog of exopolyphosphatase/guanosine pentaphosphate phosphohydrolase, regulates tick cell apoptosis.

Microbiology spectrum·2025
Same author

Modular Synthesis of 3,3-Disubstituted Azetidines via Azetidinylation Reagents.

The Journal of organic chemistry·2025
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
查看所有相关文章

相关实验视频

Updated: Jun 14, 2025

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
09:28

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

15.0K

细胞培养方法 细胞培养方法

Xin-Ru Wang1,2,3, Benjamin Cull4

  • 1Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, NY, USA. wangxin@upstate.edu.

Methods in molecular biology (Clifton, N.J.)
|June 13, 2025
PubMed
概括
此摘要是机器生成的。

细胞系,如Ixodes scapularis ISE6,为研究传播疾病和病原体提供了至关重要的模型. 这些细胞系需要特定的培养条件才能成功繁殖和研究应用.

关键词:
L-15B 中等水平的矿物质和维生素库存 矿物质和维生素库存存储 存储 存储 存储滴滴细胞线路的细胞线路.

更多相关视频

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

46.2K
Real-time Live Imaging of T-cell Signaling Complex Formation
10:31

Real-time Live Imaging of T-cell Signaling Complex Formation

Published on: June 23, 2013

13.8K

相关实验视频

Last Updated: Jun 14, 2025

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
09:28

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

15.0K
Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

46.2K
Real-time Live Imaging of T-cell Signaling Complex Formation
10:31

Real-time Live Imaging of T-cell Signaling Complex Formation

Published on: June 23, 2013

13.8K

科学领域:

  • 兽医昆虫学 兽医昆虫学
  • 寄生虫学的寄生虫学
  • 细胞生物学 细胞生物学

背景情况:

  • 细胞系对于研究和传播疾病至关重要.
  • 它们可以研究病原体相互作用和病原体传播.
  • 现有的细胞系往往需要专门的,高维护的培养条件.

研究的目的:

  • 用ISE6细胞系描述基本的细胞培养方法.
  • 为了展示ISE6细胞的形态特征.
  • 为了证明细胞培养用于病原体培养的适应性.

主要方法:

  • 利用ISE6,一个Ixodes scapularis胚胎细胞系.
  • 在特定,优化的条件下培养细胞.
  • 确定的细胞系的被记录的形态特征.

主要成果:

  • 成功培养并维持ISE6细胞系.
  • 描述了ISE6细胞的独特形态特征.
  • 证明了适应细胞培养用于病原体研究的可行性.

结论:

  • 细胞系,以ISE6为例,是和传播疾病研究的宝贵工具.
  • 优化的培养方法对于保持细胞系生存能力至关重要.
  • 这种方法支持体外培养传播的病毒和细菌病原体.