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Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
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现场RNA杂交,可视化传播病毒

Allen T Esterly1, Erin S Reynolds2,3,4, Charles E Hart5

  • 1Section of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, New Haven, CT, USA.

Methods in molecular biology (Clifton, N.J.)
|June 13, 2025
PubMed
概括

这项研究详细介绍了一种新的RNA in situ杂交 (ISH) 方法,用于在感染组织中可视化Powassan病毒 (POWV) RNA. 该技术使用商业套件来精确检测细胞架构内的病毒RNA.

关键词:
脑子 脑子 脑子 脑子历史学 历史学 历史学在POWVV中,我们可以看到POWV.在位杂交RNA在位杂交RNA

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相关概念视频

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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