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调整核酸载荷的共价有机框架:朝着创建一个层次化的人工细胞

Yiming Wang1,2, Chen Wang1,2, Yunlong Zheng3,4

  • 1State Key Laboratory of Green Biomanufacturing, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.

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概括

研究人员使用共价有机框架 (COF) 创建了具有核结构的先进人工细胞. 这种方法使得复杂的DNA封装和无细胞转录/翻译能够用于新生物系统.

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科学领域:

  • 合成生物学
  • 材料科学
  • 生物技术

背景情况:

  • 人工细胞的自下而上的构建对于生命科学和应用至关重要.
  • 现有的单一方法往往会产生过于简单的结构.
  • 需要先进的层次结构来实现更复杂的人工细胞功能.

研究的目的:

  • 开发一种用于构建具有核状结构的人工细胞的新策略.
  • 使用共价有机框架 (COF) 进行DNA封装和分离.
  • 在这些人造核中整合无细胞转录和翻译 (CFTT).

主要方法:

  • 用于线性和循环DNA封装的时空策略的共价有机框架 (COF).
  • 采用模板牺牲方法 (COFcap-1) 在COF中封装圆形质粒.
  • 在COF结构中集成的无细胞转录和翻译系统 (CFTT).
  • 在脂质体内构建人工核逻辑门和封装的COF-DNA复合体.

主要成果:

  • 使用COF成功构建了线性和圆形DNA的核结构.
  • 证明了COF孔径和微环境对CFTT效率的关键作用.
  • 设计了三个人工核逻辑门,实现了人工细胞器之间的信息传输.
  • 在脂质体内成功封装了DNA载荷的COF,形成了等级化的人工细胞.

结论:

  • 这项研究提出了一种使用多孔COF制造高度结构化的人工细胞的新方法.
  • 开发的基于COF的核结构提高了DNA封装和CFTT的效率.
  • 这项工作为构建各种应用的复杂,层次化的人工细胞开辟了新的途径.