Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Water-Soluble Viscosity-Activated Molecular Rotor for <i>In Situ</i> Imaging of Liver Damage in the NIR-II Window.

ACS sensors·2026
Same author

Epidemiological characteristics and transmission dynamics of COVID-19 outbreaks in China: a four-year retrospective analysis using extended SEIR models.

BMC public health·2026
Same author

Synergistic Interaction Between <i>Kazachstania humilis</i> and <i>Fructilactobacillus sanfranciscensis</i> Modulates Metabolic Reprogramming to Enhance Mantou Functionality in Liquid Sourdough.

Foods (Basel, Switzerland)·2026
Same author

Unveiling the Hidden Resistome: A Comprehensive Risk Assessment of Latent Antibiotic Resistance Genes in China's Wastewater.

Environmental microbiology·2026
Same author

Oxidative stress mechanisms and potential biomarkers of methyl acetate poisoning: a urinary metabolomics study in rat model and human occupational cohort.

Frontiers in molecular biosciences·2026
Same author

The role of NINJ1 in diseases.

Cell death discovery·2026
Same journal

Invaders taking over-Mollusc faunal change in volcanic barrier lakes of the Albertine Rift biodiversity hotspot.

PloS one·2026
Same journal

AI-driven molecular diversification and ligand-based optimization of macitentan derivatives targeting VEGFR1 and endothelin signaling pathways.

PloS one·2026
Same journal

Performance patterns and records in the world aquatics masters championships: Where do the most frequently represented nations among the top-ten masters swimmers come from?

PloS one·2026
Same journal

Modeling diurnal Temperature-Rainfall relationships under multicollinearity using PLS-SEM: A case study of Ghana.

PloS one·2026
Same journal

Organizational culture, social capital, and emergency capacity in primary healthcare institutions: A cross-sectional structural equation modeling study comparing ordinary and older communities.

PloS one·2026
Same journal

Impact of kidney function on the metabolome in the general population.

PloS one·2026
查看所有相关文章

相关实验视频

Updated: Jun 16, 2025

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.1K

快速光稳扩展显微镜使用QDots和解卷点.

Loku Gunawardhana1, Wilna Moree2, Jiaming Guo1

  • 1University of Houston, Department of Electrical and Computer Engineering, Houston, Texas, United States of America.

PloS one
|June 13, 2025
PubMed
概括
此摘要是机器生成的。

扩展显微镜 (ExM) 提高了分辨率,但降低了信号. 这项研究引入了一种快速,光稳定的量子点标记协议,用于广场3D成像,增强ExM中的信号和速度.

更多相关视频

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

9.7K
Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
08:16

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Published on: February 7, 2025

397

相关实验视频

Last Updated: Jun 16, 2025

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.1K
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

9.7K
Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
08:16

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Published on: February 7, 2025

397

科学领域:

  • 显微镜的使用方法
  • 生物技术是生物技术.
  • 生物物理学的生物物理.

背景情况:

  • 扩展显微镜 (ExM) 通过物理扩展样本来提高成像分辨率.
  • ExM的信号稀释和缓慢的获取限制了它的可扩展性.
  • 现有的方法在光漂白和速度方面遇到了困难.

研究的目的:

  • 为扩展显微镜 (ExM) 开发一种快速,光稳定的成像协议.
  • 为了克服信号稀释并提高ExM的采集速度.
  • 通过ExM实现可扩展的3D成像.

主要方法:

  • 开发了一个与ExM兼容的量子点 (QDot) 标签协议.
  • 利用广场成像来更快地获取3D图像.
  • 应用的解卷利用从ExM增加了样本透明度.

主要成果:

  • 与传统染料相比,QDot标签提供了增强的光稳定性.
  • 广场成像显著改善了信号噪声比 (SNR) 和采集速度.
  • 使用广场成像的解卷方式大大减少了3D采集时间.

结论:

  • 使用QDot增强的广场成像协议减轻了ExM的局限性.
  • 这种方法使得大型ExM样本的快速,高SNR3D成像成为可能.
  • 该方法为先进的显微镜应用提供了一个可扩展的解决方案.