一个酸化驱动的无处不在开关微调了植物免疫中的Alfin类7诱导的ROS信号
Dingliang Zhang1, Xinyu Zhang1, Zhiyan Wen1
1State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Science advances
|June 13, 2025
概括
植物免疫包括平衡生长和防御. 这项研究揭示了Alfin-like 7 (AL7) 蛋白质水平是如何通过ubiquitination控制的,防止过度反应性氧物种 (ROS) 在防御反应期间积累.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物免疫力 植物免疫力
- 反应性氧物种发出信号.
背景情况:
- 维持活性氧物种 (ROS) 稳态对于植物生长与防御平衡至关重要.
- 在植物免疫过程中控制ROS积累的转录因子 (TFs) 的调节尚不清楚.
研究的目的:
- 阐明植物免疫反应期间控制ROS恒温的TF活性调节机制.
- 调查阿尔芬类7 (AL7) 和其调节剂在N介导免疫中的作用.
主要方法:
- 研究了Alfin-like 7 (AL7) 和无素蛋白联酶E3成分N-recognin 7 (UBR7) 之间的相互作用.
- 分析了由UBR7.7介导的AL7的泛化和蛋白质体降解.
- 研究了AL7酸化对其与UBR7相互作用以及随后的稳定性的影响.
主要成果:
- UBR7直接与AL7相互作用,并在lysine-20中调解其无化,将其定位为蛋白质体降解.
- UBR7在AL7的上游作用,在N介导防御过程中减少AL7诱导的ROS积累.
- 在174血清中对AL7的酸化增强了它与UBR7的相互作用,促进了ubiquitination并降低了AL7的稳定性.
结论:
- 一种涉及AL7酸化和UBR7介导的全域化的新型机制调节了植物免疫期间的TF稳定性和ROS积累.
- 这种调节途径确保精确控制ROS信号,防止过度活跃的防御反应和维持平衡.
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