Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

5.4K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.4K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026
Same author

Correcting curvature in micromirror-based spatial light modulators with a microlens array.

Optics express·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

bioRxiv : the preprint server for biology·2026
Same author

Perturbative Fourier ptychographic microscopy for fast quantitative phase imaging.

Optics express·2025
Same author

Space-time reconstruction for lensless imaging using implicit neural representations.

Optics express·2025
Same author

Ring deconvolution microscopy: exploiting symmetry for efficient spatially varying aberration correction.

Nature methods·2025
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
查看所有相关文章

相关实验视频

Updated: Jun 15, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.9K

结构化照明光显微镜的样本运动.

Ruiming Cao, Guanghan Meng, Laura Waller

    Optics letters
    |June 13, 2025
    PubMed
    概括
    此摘要是机器生成的。

    这项研究引入了一种新的超高分辨率显微镜技术,使用单点光照明和样本运动. 该方法从标准光图像中计算重建高分辨率图像,从而推进光学成像能力.

    更多相关视频

    Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
    09:59

    Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

    Published on: May 3, 2013

    17.9K
    Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography
    08:20

    Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography

    Published on: October 25, 2021

    3.3K

    相关实验视频

    Last Updated: Jun 15, 2025

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    8.9K
    Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
    09:59

    Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

    Published on: May 3, 2013

    17.9K
    Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography
    08:20

    Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography

    Published on: October 25, 2021

    3.3K

    科学领域:

    • 显微镜的使用方法
    • 光学成像技术的成像
    • 计算生物学 计算生物学

    背景情况:

    • 结构化照明显微镜 (SIM) 通过从多个模式计算重建图像来实现超分辨率.
    • 传统的SIM需要复杂的照明设置和精确的对齐.

    研究的目的:

    • 开发一种使用单一斑点图案的替代超分辨率显微镜方法.
    • 为了利用固有的样本运动来编码超分辨率的信息.

    主要方法:

    • 获取多个原始光图像与单一的光斑照明模式.
    • 对样本运动和超分辨率图像的联合计算估计.
    • 通过模拟数据和实验成像验证.

    主要成果:

    • 拟议的单光照明方法的可行性证明.
    • 通过分析样本运动,成功重建超分辨率图像.
    • 对于简化超分辨率显微镜设置的潜力.

    结论:

    • 拟议的方法为实现超分辨率提供了传统SIM的可行替代方案.
    • 利用样本运动提供了一种新的方式来编码高分辨率信息.
    • 这种技术可以简化超高分辨率成像系统的要求.