来自未化蛋的pH偏移蛋白质分离物的结构和技术功能性质
Supalak Ruangprom1, Manat Chaijan1, Chantira Wongnen1
1Food Technology and Innovation Research Center of Excellence, School of Agricultural Technology and Food Industry, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Poultry science
|June 13, 2025
概括
处理pH转移会改变未化母蛋蛋白分离物 (PPI). 酸性和性处理改变了物理化学和技术功能性质,影响了粘度,稳定性和凝性,与商用全蛋粉相比.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物化学 生化学
背景情况:
- 未出的母蛋 (不育和死在中) 是一个潜在的蛋白质来源.
- 了解它们的蛋白质隔离物 (PPI) 特性对于食品应用至关重要.
- pH-shift处理是一种修改蛋白质功能的一种方法.
研究的目的:
- 调查酸性和性pH转移处理对未化母蛋的PPI的影响.
- 为了比较这些特性与商业全蛋粉 (CEP).
- 评估物理化学和技术功能特性.
主要方法:
- 蛋白质分离物 (PPI) 是从无育的 (INF) 和死在中的 (DIS) 蛋中制备的,使用酸性和性pH变化处理.
- 分析了物理化学性质 (结构,热变性,可溶性,粘度).
- 技术功能性质 (泡性,泡稳定性,乳化活性,乳液稳定性,凝) 被评估.
- 进行了风病学分析 (G',G',tan δ).
主要成果:
- 与CEP相比,PPI显示结构变化,降低SH含量,以及较低的表面疏水性.
- DIS性PPI具有最高的热变性温度 (Td).
- CEP具有更高的溶解性和泡性,而PPI (特别是酸-INF-PPI) 显示出更好的泡稳定性.
- DIS性PPI表现出优异的乳液稳定指数 (ESI).
- 与PPI凝相比,CEP形成了比PPI凝更强的凝,具有更高的水容量 (WHC).
- INF性PPI表现出最高的弹性 (G'),但CEP具有优越的机械强度.
结论:
- 处理pH转移显著影响未化卵PPI的结构和技术功能性质.
- 特定的pH处理 (例如,性DIS) 可以增强某些功能,如粘度和乳液稳定性.
- 虽然CEP在某些方面 (溶解度,凝强度) 通常优于PPI,但PPI为特定的食品应用提供了潜力,特别是在泡稳定性和弹性方面.
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