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Perturb-Multimodal:一个在完整的哺乳动物组织中进行成像和测序的综合基因查平台

Reuben A Saunders¹, William E Allen², Xingjie Pan³

¹Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA; Whitehead Institute, Cambridge, MA 02139, USA; University of California, San Francisco, San Francisco, CA 94158, USA.

|June 13, 2025

概括

这项研究介绍了Perturb-Multimodal (Perturb-Multi),一种用于将遗传干扰映射到组织中的细胞表型的新方法. 它揭示了控制肝细胞区分,蛋白质反应和肥胖症的遗传调节者.

科学领域:

细胞和分子生物学
遗传学
系统生物学

背景情况:

甲基动物的生命依赖于不同细胞类型的协调基因活动.
了解组织功能需要剖析细胞和组织表型的体内遗传控制.

研究的目的:

介绍Perturb-Multimodal (Perturb-Multi),一种新的对照成像和测序方法.
在组织中使用聚合的遗传干扰来构建大规模的多模式基因型-表型图.

主要方法:

Perturb-Multi集成成像用于个体细胞扰动识别,基因表达概况和亚细胞形态测量.
同时单细胞测序捕获了对遗传干扰的完整转录组反应.
这种方法应用于小鼠肝脏组织中的数百种遗传乱.

主要成果:

这项研究确定了控制肝细胞区分的遗传调节剂和机制.
它阐明了肝细胞中未展开的蛋白质反应的遗传控制.
使用这种多模式方法研究了肥胖症的遗传基础.

结论:

Perturb- Multimodal (Perturb- Multi) 显著加速了复杂细胞和组织生理学的遗传基础的发现.
生成的数据可以作为细胞功能的机器学习模型的关键训练数据集.

关键词:

美国海军海军肝细胞应激反应在体内组合查脂肪滴积累肝脏区域化机器学习形态多模式表型化多重RNA成像多重蛋白质成像 scRNA-seq 在

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