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格拉姆阴性内细菌通过宫癌中的LPS-TLR4/MAPK信号通路介导淋巴结转移

Yaqin Liu¹, Peng Ma¹, Dongdong Liu²

¹Department of Laboratory Medicine, The Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, PR China.

The Journal of infection

|June 13, 2025

概括

像大肠杆菌这样的阴性细菌通过激活信号通路来促进宫癌 (CC) 淋巴结转移. 针对这些细菌和通路可能为CC患者提供新的治疗策略.

科学领域:

在瘤学瘤学.
微生物学微生物学
癌症转移癌症转移

背景情况:

内细菌在固体瘤中普遍存在,并影响瘤的进展.
淋巴结转移是宫癌 (CC) 死亡率的一个关键因素.
内细菌在CC淋巴转移中的作用尚不清楚.

研究的目的:

调查宫癌组织中内细菌的存在和分布.
为了确定特定的格拉姆阴性细菌对淋巴内皮细胞和转移的影响.
阐明参与细菌诱导转移的分子机制,包括信号通路和前兆转移因子.

主要方法:

16S rDNA测序和细菌培养以识别内细菌.
使用人类淋巴内皮细胞 (HLEC) 进行体外试验 (透孔,毛细管形成).
对TLR4/MAPK信号通路激活和EFNA1/EDN2表达的分析.
在小鼠模型中的体内研究使用细菌脂多糖 (LPS) 和活细菌.

主要成果:

格拉姆阴性细菌,包括大肠杆菌 (E. coli),Prevotella bivia (P. bivia) 和Fusobacterium nucleatum (F. nucleatum),在转移性CC组织中非常丰富.
这些细菌促进了HLEC毛细管的形成,并通过膜信号传递增强了瘤细胞转移.
细菌脂多糖 (LPS) 激活了TLR4/MAPK通路,从而调高了前列腺转移因子EFNA1和EDN2.
在体内,LPS和活体大肠杆菌显著增加了小鼠的淋巴结转移.

结论:

格拉姆阴性细菌 (大肠杆菌,P. bivia,F. nucleatum) 在促进宫癌淋巴结转移方面起着因果作用.
TLR4/MAPK信号通路和EFNA1/EDN2因子是细菌诱导转移的关键媒介.
准这些细菌及其信号通路为宫癌提供了潜在的治疗策略.

关键词:

宫癌是发生在宫癌的原因之一.在LPS中使用LPS.这就是MAPKK的意思.格拉姆阴性细菌是一种细菌.内细菌是一种口内细菌.

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