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优化Trichophyton indotineae的检测及其在澳大利亚大型实验室的流行率

Kyra Y L Chua¹, Catriona L Halliday², Amber Mason³

¹Department of Microbiology, Dorevitch Pathology, Heidelberg, Vic, Australia; Department of Infectious Diseases, University of Melbourne, the Peter Doherty Institute for Infection and Immunity, Melbourne, Vic, Australia; Department of Infectious Diseases and Immunology, Austin Health, Heidelberg, Vic, Australia.

|June 13, 2025

概括

这项研究估计了墨尔本抗药性真菌Trichophyton indotineae的流行率,在0.6%的皮肤植物标本中发现了它. 鉴定需要内部转录间隔器 (ITS) 测序,因为表型方法对这种新出现的病原体不够.

科学领域:

医学真菌学医学真菌学
临床微生物学临床微生物学
传染性疾病传染性疾病

背景情况:

三菌 (Trichophyton indotineae) 是一种新兴的,耐药的真菌物种,导致皮肤植物病.
在研究实验室中没有报告过T. indotineae的先前隔离.
准确识别T. indotineae对于有效的治疗和公共卫生监测至关重要.

研究的目的:

在澳大利亚墨尔本的皮肤植物标本中估计Trichophyton indotineae的流行率.
评估用于检测和识别T. indotineae. 的实验室实践.
为了突出表型方法在识别该物种的局限性.

主要方法:

在2024年1月至6月期间收集的皮肤和头发样本中分析了202个Trichophyton分离物.
通过显微镜和培养检测皮肤菌,然后进行常规的表型鉴定.
内部转录间隔器 (ITS) 区域测序,用于确定尿酶负或不确定的隔离物.

主要成果:

最常见的物种是Trichophyton rubrum (63.4%),其次是T. interdigitale (18.8%) 和T. indotineae (6.4%).这些物种是最常见的物种.
鉴定出了13种T. indotineae分离体,估计患病率为0.6% (95%CI为0.3-1.0).
所有T. indotineae分离物最初在表型上被误认为T. interdigitale或其他Trichophyton物种.

结论:

现型方法不足以识别Trichophyton indotineae;需要进行ITS测序.
虽然T. indotineae在这个澳大利亚队列中似乎不常见,但需要进一步的研究来确定其真正的流行率.
改进的实验室诊断对于检测这种新兴的耐药真菌至关重要.

关键词:

澳大利亚澳大利亚澳大利亚这种植物是Trichophyton indotineae.抗真菌药物耐药性抗真菌药物耐药性皮肤质的皮肤类植物.特尔比纳非因 (terbinafine) 是一种药物.一个小小的青春.

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