Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Riboswitches01:56

Riboswitches

8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Types of RNA01:23

Types of RNA

63.4K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.4K
Initiation of Translation02:33

Initiation of Translation

31.9K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
31.9K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

29.4K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.4K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

2.8K
2.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

<i>Sheathia shaoguanensis</i> sp. nov. (Batrachospermales, Rhodophyta), a new freshwater red algal species from South China.

PhytoKeys·2026
Same author

[<i>Tongyuan</i> acupuncture for postherpetic neuralgia: a randomized controlled trial].

Zhongguo zhen jiu = Chinese acupuncture & moxibustion·2026
Same author

Aging Enhances Serotonergic Signaling via 5-HT<sub>7</sub> Receptors Underlying Mechanical Alloknesis.

Aging cell·2026
Same author

Multi-omics analysis reveals mechanisms of Qingying granules in treating porcine warm disease: dosage optimization and systems biology insights.

BMC veterinary research·2026
Same author

Amino-acids-mTORC1-driven DDA1 phosphorylation promotes DNA repair and glioblastoma progression.

Cell communication and signaling : CCS·2026
Same author

Structural insights into the specificity of adaptor proteins Dok5 and Dok6 Docking to Trk receptors.

Cell communication and signaling : CCS·2026

相关实验视频

Updated: Jun 16, 2025

Production of A SARS-CoV-2 Virus like Particle System to Investigate Viral Life Cycles In Vitro
09:26

Production of A SARS-CoV-2 Virus like Particle System to Investigate Viral Life Cycles In Vitro

Published on: June 6, 2025

71

干环结合蛋白促进SARS-CoV-2复制通过-1编程的核糖体框架转移.

Tanxiu Chen1,2,3, Ruimin Zhu1, Tingfu Du2

  • 1State Key Laboratory of Respiratory Health and Multimorbidity, National Center of Technology Innovation for Animal Model, Key Laboratory of Pathogen Infection Prevention and Control (Peking Union Medical College), Ministry of Education, Institute of Laboratory Animal Science, CAMS & PUMC, Beijing, China.

Signal transduction and targeted therapy
|June 13, 2025
PubMed
概括

干环结合蛋白 (SLBP) 促进SARS-CoV-2的框架转移,这是一个关键的病毒过程. 这一发现确定了SLBP作为COVID-19治疗的潜在治疗标.

更多相关视频

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.0K
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

4.9K

相关实验视频

Last Updated: Jun 16, 2025

Production of A SARS-CoV-2 Virus like Particle System to Investigate Viral Life Cycles In Vitro
09:26

Production of A SARS-CoV-2 Virus like Particle System to Investigate Viral Life Cycles In Vitro

Published on: June 6, 2025

71
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.0K
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

4.9K

科学领域:

  • 病毒学 病毒学
  • 分子生物学分子生物学
  • 宿主-病原体相互作用

背景情况:

  • 严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 依赖于-1编程的核糖体框架转移 (-1 PRF) 进行多蛋白质合成.
  • 调节SARS-CoV-2 -1 PRF的宿主因素在很大程度上仍未确定,限制了治疗策略.

研究的目的:

  • 为了识别与SARS-CoV-2 -1 PRF RNA相互作用的宿主蛋白质.
  • 研究已识别的宿主蛋白在病毒复制和框架转移中的作用.
  • 探索COVID-19的潜在治疗点.

主要方法:

  • RNA拉下测试与质谱学相结合,以确定相互作用的主体蛋白质.
  • 深度学习预测 (PrismNet) 对于RNA与蛋白质结合的概率.
  • 电泳运动移位测试 (EMSA) 和RNA拉下测试以确认直接结合.
  • 小光显微镜在现场杂交 (smFISH) 用于定位化研究.
  • 在体外翻译系统以评估移效率.

主要成果:

  • 确定了五种宿主蛋白质,包括干环结合蛋白 (SLBP),与-1 PRF RNA相互作用.
  • SLBP直接与SARS-CoV-2 -1 PRF RNA结合,特别是在干环3区域.
  • 过度表达SLBP增强-1PRF并促进病毒复制.
  • SLBP影响其他宿主因子 (FUBP3,RPS3A,RPL10A) 与-1 PRF RNA区域的结合.

结论:

  • 干环结合蛋白 (SLBP) 是一种促进SARS-CoV-2 -1 PRF的新型宿主因子.
  • SLBP与病毒RNA的相互作用对于有效的病毒复制至关重要.
  • SLBP代表了开发新型COVID-19治疗方法的潜在可用药物标.