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一种天然化合物准的主转录因子的相分离调节了真菌埃尔戈斯特醇的恒常性

Huiyuan Wang^1,2, Yuhua Wang³, Jingrui Wang^1,2

¹National Key Laboratory of Rice Biological Breeding, Zhejiang University, Hangzhou, 310058, China.

Science China. Life sciences

|June 13, 2025

概括

研究人员发现了一种新的方法,真菌使用转录因子FfSR控制厄戈斯特醇水平. 这一发现可能会导致更好的治疗由Fusarium fujikuroi引起的米病.

科学领域:

菌类学菌类学是指菌类学.
分子生物学分子生物学
生物化学生物化学

背景情况:

雌激素稳定对于真核生物来说至关重要,但在致病性真菌中人们对其了解甚少.
富沙 (Fusarium fujikuroi) 致使米病,影响全球农业.

研究的目的:

为了识别Fusarium fujikuroi中的ergosterol平衡的关键调节者.
阐明这种真菌病原体中厄戈斯特醇调节的分子机制.
探索针对厄戈斯特醇生物合成的潜在治疗策略.

主要方法:

基因删除研究评估FfSR.的作用.
对厄戈斯特醇水平和对醇真菌杀菌剂敏感性的分析.
调查FfSR相位分离和转录活性,以响应厄戈斯特醇水平.
识别抑制FfSR功能的小分子.

主要成果:

在F. fujikuroi中,FfSR被确定为对ergosterol恒温的关键转录因子.
删除FfSR导致埃尔戈斯特的降低,并增加对醇杀菌剂的敏感性.
亚醇诱导的厄戈斯特醇枯竭会触发FfSR相分离和基因激活,而高水平的厄戈斯特醇会抑制它.
纳塔米辛被确定为FfSR的直接抑制剂,阻断其相位分离和转录活性.

结论:

菌病原体通过转录因子相分离来调节厄戈斯特的恒常性.
用小分子准FfSR可能会增强对致病性Fusarium的阿佐尔真菌杀菌剂疗效.
这项研究揭示了控制真菌固醇生物合成的新机制,并为疾病管理提供了潜力.

关键词:

富萨里乌姆 (Fusarium) 是一个富士基罗伊 (fujikuroi) 的植物.抗真菌药物是一种抗真菌药物.埃尔戈斯醇的恒温作用液态-液态相隔离方法液态相隔离方法转录因子的转录因子

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