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Updated: Jun 16, 2025

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通过Strep/FLAG (SF) -TAP标签对病毒-宿主蛋白质复合体进行并列亲和净化

Zunlin Yang1, Chunfu Zheng2

  • 1School of Medicine, Johns Hopkins University, Baltimore, MD, USA.

Methods in molecular biology (Clifton, N.J.)
|June 14, 2025
PubMed
概括

链杆菌/FLAG-TAP (SF) 标签通过消除蛋白酶分裂的需要,简化了蛋白质复合体的隔离,使哺乳动物细胞中病毒和宿主相互作用的有效净化成为可能.

关键词:
哺乳动物细胞是哺乳动物细胞.非特定蛋白质的减少.蛋白酶没有分裂,没有裂变.蛋白质蛋白质相互作用双重亲和性净化 (TAP) 是一种方法.病毒与主机的互动

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