使用增强的LC-MS分离与五黄 phenylpropyl-functionalized 列和基于预测的MS/MS频谱注释的水友性代谢物的非目标分析
Masaru Sato1, Kazutaka Ikeda2,3
1Laboratory of Biomolecule Analysis, Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
概括
这项研究引入了一种改进的液体色谱-质谱法 (LC-MS) 方法,使用五烯烯 (PFP) 列进行全面的水友性代谢物分析. 开发的注释策略增强了检测到的代谢物的生物相关性识别.
科学领域:
- 代谢学 代谢学 代谢学
- 分析化学 分析化学
- 生物化学 生化学
背景情况:
- 针对广泛的代谢组分析在生物学研究中至关重要,但在全面的水友性代谢物分析中面临挑战.
- 现有的分析和信息技术需要改进,以便对初级和二级水友性代谢产物进行无针对性分析.
研究的目的:
- 开发一种先进的染色学方法来分析水友性代谢物.
- 为各种水友性代谢物建立一个有效的注释方法.
主要方法:
- 研究了基于液态染色体质谱法 (LC-MS) 的代谢组分析的五黄烯基 (PFP) 柱的性能.
- 雇佣的MS/MS对代谢物结构数据库进行光谱相似性搜索,以进行峰值注释.
- 使用水/甲醇与酸和氨酸盐的优化移动相.
主要成果:
- 在LC-MS分析过程中,PFP列改善了水友性代谢物的保留和峰值形状.
- 在54%的标准化合物中,化学结构得到了正确的注释,超过70%的化合物被确定为生物相关的.
- 对番茄果实的分析检测并注释了658和458个正离子模式的峰值,分别是正离子和负离子模式.
结论:
- 结合LC-MS方法与PFP列和增强的注释有助于全面的水友性代谢组分析.
- 这种方法推进了在生物样本中研究各种水友性代谢物的研究.
更多相关视频
11:00Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
22.4K
09:04Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
Published on: April 18, 2019
12.4K
相关概念视频
01:21Mass Spectrometry: Complex Analysis
736
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
736
01:33Peptide Identification Using Tandem Mass Spectrometry
6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K