转录和酶分析显示了氨和酸盐在Ruditapes philipparum上的协同毒性
Ming Cong1, Yixian Yu1, Zhaoshun Li1
1Ocean School of Yantai University, Yantai 264005, PR China.
Ecotoxicology and environmental safety
|June 14, 2025
概括
对氨和亚酸的联合暴露会导致的协同毒性. 这会扰乱新陈代谢,导致细胞损伤,对海洋双鱼养殖业构成重大威胁.
科学领域:
- 环境毒理学环境毒理学
- 海洋生物学 海洋生物学
- 分子毒理学分子毒理学
背景情况:
- 之前的研究表明,联合氨 (NH3-N) 和化 (NO2-N) 暴露会改变的代谢物和酶.
- 的NH3-N和NO2-N联合暴露的确切毒理机制尚不清楚.
研究的目的:
- 阐明Ruditapes philipparum中NH3-N和NO2-N联合暴露的协同毒性背后的分子机制.
- 确定单一和联合化合物暴露影响的关键途径和细胞过程.
主要方法:
- 鲁迪塔佩斯菲利帕拉姆 (Ruditapes philipparum) 被暴露在单一或组合的NH3-N和NO2-N中,持续了7天.
- 使用比较转录组分析来识别差异表达基因 (DEG).
- 进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.
主要成果:
- 联合暴露显示出比单个暴露更高的毒性,与线粒体损伤,炎症和免疫反应途径的早期激活.
- 诱导了协同毒性通路,破坏了碳水化合物和蛋白质代谢,能源生产,并导致细胞分解.
- 线粒体损伤通过增加的酶活性 (例如,α-KGDH,SDH) 和改变的氧化还原参数 (例如,SOD,CAT,GSH) 得到证实.
结论:
- 在环境相关度下,NH3-N和NO2-N在R. philipparum中表现出协同毒性.
- 线粒体功能障碍是NH3-N和NO2-N联合毒性的关键机制.
- 这种协同毒性对海洋双鱼水产养殖和生态系统健康构成重大威胁.
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