NSUN2通过甲基化基因酶3-like-1mRNA促进PM2.5诱导的上皮层-介质细胞过渡
Mengyao Shao1, Chunjiang Li1, Jingyi Li1
1Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Journal of hazardous materials
|June 14, 2025
概括
暴露于PM2.5会增加NSUN2和CHI3L1mRNA甲基化,导致肺纤维化. NSUN2 缺乏症可以抵消这种情况,为呼吸系统疾病提供治疗见解.
科学领域:
- 环境健康 环境健康
- 分子生物学分子生物学
- 呼吸系统医学 呼吸系统医学
背景情况:
- 胆酶3样-1 (CHI3L1) 是呼吸系统疾病的关键生物标志物和治疗标.
- 在PM2.5诱导的肺纤维化中CHI3L1调节的分子机制在很大程度上是未知的.
研究的目的:
- 阐明NSUN2和m5C甲基化在PM2.5暴露下调节CHI3L1表达中的作用.
- 调查NSUN2对PM2.5诱导的肺纤维化和上皮层-介质细胞过渡 (EMT) 的影响.
主要方法:
- 在PM2.5处理的肺组织和MLE-12细胞中分析NSUN2表达和CHI3L1mRNAm5C甲基化.
- 研究CHI3L1mRNA的NSUN2介导的m5C甲基化及其通过ALYREF的细胞质运输.
- 评估NSUN2缺乏对PM2.5诱导的肺纤维化和EMT in vivo和in vitro的影响.
主要成果:
- 暴露于PM2.5导致CHI3L1mRNA的NSUN2表达和m5C甲基化增加.
- NSUN2在3'UTR中催化CHI3L1mRNA的m5C甲基化,促进其依赖ALYREF的细胞质运输.
- 由于NSUN2缺乏,可以缓解PM2.5诱导的CHI3L1 m5C甲基化和EMT.
结论:
- 通过NSUN2介导的CHI3L1的m5C甲基化是PM2.5诱导的肺纤维化的一个关键机制.
- 准NSUN2可能为缓解PM2.5相关的呼吸道疾病提供一种新的治疗策略.
相关概念视频
MicroRNAs
21.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.2K
Epigenetic Regulation
31.0K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.0K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
mRNA Stability and Gene Expression
5.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.6K


