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作为外源前体,内生细菌的氨酸和跨氨酸参与了Gloriosa superbaba中调节菌素生物合成途径的外源前体

Pradeep Semwal¹, Radha Shivhare¹, Bsudev Majhi¹

¹Microbial Technologies Division, Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.

Plant physiology and biochemistry : PPB

|June 14, 2025

概括

内生菌细菌通过促进植物生长和关键代谢前体,显著增强Gloriosa superba中的菌素生产. 这一发现为制药应用提供了一种可持续的方法.

科学领域:

植物科学植物科学
微生物学微生物学
生物化学生物化学

背景情况:

植物内存的微生物,即内生菌,可以影响宿主生理和代谢物生产.
格洛里奥萨 (Gloriosa superba) 是一种有价值的药用植物,但其活性化合物 - - 素 (colchicine) 的生产可能受到限制.
了解内生植物与植物的相互作用对于优化药用植物种植至关重要.

研究的目的:

调查内菌对Gloriosa superba.的生长和菌素生物合成的影响.
为了确定关键的前体和代谢途径,涉及到内细胞介导的菌素生产.
探索内生菌菌在可持续农业和制药应用中的潜力.

主要方法:

对内细胞培养过物的代谢物分析,以确定前体.
用Bacilli治疗Gloriosa superba植物,并与未经处理的植物进行比较.
分析植物生长,素含量,营养吸收和酶活性 (PAL,NMT,CYP450,OMT).
超基因组分析,以评估内生菌微生物组的变化.

主要成果:

菌治疗显著增加了Gloriosa superba植物的生长和菌素含量.
在接受治疗的植物中观察到,基本营养素 (Fe,Zn,Cu,Mn) 的吸收增加.
诱导了与菌素生物合成基因相关的酶活性,以及对关键前体如乙醇,氨酸,跨酸和酸的上调调节.
甲基因组分析显示了Bacillus物种的转变,并证实了生物合成途径的上调.

结论:

菌在增强Gloriosa superba生长和菌素生物合成方面发挥着重要作用.
细菌改善营养吸收,调节酶活性和微生物群落,导致代谢产量的增加.
这项研究为在可持续农业中利用内植物和开发新型药物应用提供了基础.

关键词:

细菌是一种细菌.科尔奇辛 (Colchicine) 是一种可口药.它们是内植物 (Endophyte).超级宝贝G. 超级宝贝其次代谢物二次代谢物.

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