一种用于确定传输效率的方法激光消光诱导合等离子体质谱用于组织分析
Jaromír Stráník1, Vilém Svojanovský1, Julie Weisová2
1Department of Chemistry, Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic.
Analytical chemistry
|June 15, 2025
概括
本研究提出了一种新的方法来测量使用光纳米粒子进行激光剥离-诱导合等离子体质谱的运输效率 (TE). 该技术提供了精确的TE确定,这对于可靠的纳米粒子分析至关重要.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
背景情况:
- 激光切除-诱导合等离子体质谱法 (LA-ICP-MS) 中的单颗粒模式可以同时绘制粒子映射和尺寸.
- 运输效率 (TE) 是影响LA-ICP-MS中纳米粒子检测和表征准确性的关键参数.
- 精确的TE测定对于可靠的纳米粒子分析和相关应用至关重要.
研究的目的:
- 在LA-ICP-MS中引入和验证一种用于精确确定运输效率 (TE) 的新方法.
- 评估拟议方法在不同激光波长和度的适用性.
- 为了确定在单粒子LA-ICP-MS中控制TE的因素.
主要方法:
- 开发了一种新的TE确定方法,利用嵌入在凝中的上转化纳米粒子 (UCNPs) 的计数.
- 使用光显微镜量化了被消化和运输的UCNP的数量.
- 该方法使用2940nm,213nm和193nm激光消光系统进行了测试.
主要成果:
- 拟议的方法通过2940nm激光实现了几乎定量化的纳米颗粒脱吸,TE主要受到剥离细胞设计和气溶输送的影响.
- 对于213nm激光器,在低流量下不完整的脱落和潜在的重新定位需要仔细考虑,以确保TE测量的可变性.
- 193纳米激光诱导了纳米粒子分解,导致基线噪声增加和灵敏度降低,排除了使用该方法来确定TE的可能性.
结论:
- 开发的方法提供了一种通用方法,用于在单颗粒LA-ICP-MS中精确地确定TE.
- 该方法的有效性取决于激光波长和流动性,在2940nm观察到的最佳性能.
- 了解和控制TE对于使用LA-ICP-MS推进纳米粒子表征至关重要.
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