由酵母Candida tropicalis产生的氧化糖还原酶,使用RSM优化的水性双相系统进行净化
Leanderson Tulio Marques Lemos1, Lorena Lucena de Medeiros2, Normando Ribeiro-Filho1
1Postgraduate Program in Food Science and Technology, Federal University of Paraíba, Campus I - Technology Center, CEP, 58051-900 João Pessoa, PB, Brazil; Postgraduate Program in Chemical Engineering, Federal University of Paraíba, Campus I - Technology Center, CEP, 58051-900 João Pessoa, PB, Brazil.
Food chemistry
|June 15, 2025
概括
这项研究优化了使用南瓜果废弃物 (CAW) 水解剂的氧化糖减少酶 (XR) 净化. 使用水性双相系统 (ATPS) 的响应表面方法 (RSM) 提高了XR净化效率,降低了成本.
科学领域:
- 生物技术是生物技术.
- 生物化学 生物化学
- 酶工程是什么? 酶工程是什么?
背景情况:
- 甘菌酵母产生的西立醇 (XP) 是一个有价值的过程.
- 与氧化糖还原酶 (XR) 酶生产和净化相关的高成本限制了XP的可扩展性.
- 果仁果废弃物 (CAW) 为XR生产提供了低成本的基材,但净化仍然是一个挑战.
研究的目的:
- 使用水性双相系统 (ATPS) 优化氧化糖还原酶 (XR) 净化.
- 应用响应表面方法 (RSM) 来优化ATPS参数以实现高效的XR净化.
- 为了降低XR生产的整体成本,以增强利醇合成.
主要方法:
- 果仁果废物 (CAW) 被水解以准备酵母发酵的基质.
- 使用Candida酵母 (CCT 1516) 制造了XR.
- 使用响应表面方法 (RSM) 来优化ATPS成分,使用不同的聚乙烯甘醇 (PEG) 和酸 (PP) 度.
主要成果:
- 在40小时内,获得的最大西利托度为15.4g/L.
- 在28小时获得0.4U/mg的最大XR活性.
- RSM分析表明,PEG对XR净化产生了积极的影响,而PP没有显著影响.
结论:
- 使用RSM的优化ATPS可以有效地从CAW水解物中净化XR.
- 开发的方法为XR净化提供了一种具有成本效益的方法,可能降低西利生产成本.
- 进一步的研究可以探索将这种优化净化过程扩展到工业应用.
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