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分子陪伴者:一种革命性的方法来提高来自细菌和酵母系统的复合mAbs的溶解度

Niharika Ashish Kulkarni¹, Prabir Kumar Das¹, Arjun P¹

¹Biochemical Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.

Protein expression and purification

|June 15, 2025

概括

分子陪伴剂通过改善治疗单克隆抗体 (mAbs) 的折叠性和溶解性来增强大肠杆菌和酵母中的重组蛋白质生产. 工程策略进一步优化生物制药制造的mAb质量.

科学领域:

生物技术和生物制药制造业生物技术和生物制药制造业
分子生物学和蛋白质工程分子生物学和蛋白质工程

背景情况:

重组蛋白质表达对于产生治疗性蛋白质至关重要,但蛋白质错折和聚合等挑战仍然存在,特别是在诸如大肠杆菌 (Escherichia coli) 等宿主中.
分子伴侣对于适当的蛋白质折叠,溶解性和稳定性至关重要,解决了重组蛋白质生产的关键局限性.

研究的目的:

审查分子陪伴者和工程策略在增强重组单克隆抗体 (mAbs) 生产和质量的作用.
为了比较细菌 (大肠杆菌) 和酵母菌 (Saccharomyces cerevisiae,Pichia pastoris) 表达系统中的陪伴式辅助折叠方法.

主要方法:

在大肠杆菌中探索伴侣系统 (例如GroEL/GroES,DnaK/DnaJ/GrpE,Skp) 以改善mAb折叠和溶解性.
酵母护航系统 (Hsp70-90,TRiC,RAC/NAC) 的分析,用于全长和零碎的mAb折叠.
共同表达,分泌途径,工程宿主菌株,人工伴侣 (纳米颗粒) 和mRNA工程的检查.

主要成果:

分子陪伴剂显著改善了E. coli和酵母系统中复合mAbs的折叠,溶解性和活性.
协同表达,工程宿主,以及人工陪伴者和mRNA工程等新的方法提供了进一步的优化潜力.
对比分析突出了不同表达平台上的多样化的护送应用程序.

结论:

陪伴者集成和先进的工程策略对于克服重组 mAb 生产中的瓶至关重要.
优化伴侣功能和宿主系统对于推动生物制药制造和治疗蛋白质的工业应用至关重要.

关键词:

细菌细菌是一种细菌.碎片化的抗体碎片化的抗体分子的伴侣是分子的伴侣.单克隆抗体是一种单克隆抗体.可溶性溶解度可溶性酵母酵母是一种酵母.

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