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Jaymi January1, Olivier Zwaenepoel2, Jan Gettemans2

  • 1SensorLab (University of the Western Cape Sensor Laboratories), 4th Floor Chemical Sciences Building, University of the Western Cape, Robert Sobukwe Road, Bellville, 7535, Cape Town, South Africa; Department of Biomolecular Medicine, Nanobody Lab, Faculty of Medicine and Health Sciences, Ghent University, Tech Lane Ghent Science Park 75, 9052, Ghent, Belgium.

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概括

一种新的免疫传感器可以检测巨细胞封闭蛋白 (CapG),这是一种癌症生物标志物. 这种敏感的工具,使用矿标记的纳米体,显示了早期癌症查的希望,特别是胃,乳腺和卵巢癌.

关键词:
癌症疾病的生物标志物电化学免疫传感器 电化学免疫传感器巨细胞封闭蛋白 (CapG) 是一种巨细胞封闭蛋白.基于纳米体的免疫传感器.矿石纳米材料是一种矿石纳米材料.

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科学领域:

  • 生物医学工程 生物医学工程
  • 纳米技术纳米技术
  • 癌症生物标志物 癌症生物标志物

背景情况:

  • 巨细胞封闭蛋白 (CapG) 的过度表达与乳腺,卵巢和胃癌有关.
  • CapG是一种潜在的新生物标志物,用于早期癌症检测.
  • 对CapG的敏感和选择性检测方法对于癌症诊断至关重要.

研究的目的:

  • 开发一种新的LiSmZrO3矿标记纳米体 (Nb) 类型的三明治免疫传感器,用于CapG检测.
  • 为了评估免疫传感器的灵敏度,选择性和稳定性,用于CapG检测.
  • 探索这种免疫传感器作为癌症查工具的潜力.

主要方法:

  • 使用抗CapG纳米体 (单抗原结合域驼类重链单抗体) 制造三明治免疫传感器.
  • 在金印刷电极上的捕获纳米体 (Nb1) 的固定,通过链路链接.
  • 使用带有LiSmZrO3矿标记的信号纳米体 (Nb2) 作为电化学氧化还原标记器.

主要成果:

  • 免疫传感器显示,在缓冲器中检测CapG的线性校准范围为0-1200 pg/mL.
  • 在杜尔贝科的酸盐缓冲盐水中,达到326.4 pg/mL的检测极限 (LOD).
  • 在人血清中,获得了1400 pg/mL的LOD,表明可用于真实样本.

结论:

  • 开发的CapG纳米体型免疫传感器提供了对CapG的敏感和选择性检测.
  • 免疫传感器表现出高稳定性,使其适合实际应用.
  • 这种新型免疫传感器通过检测升高的CapG水平,有可能成为癌症查的有效工具.