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实施高分辨率精子呼吸计,用于模拟公牛的生育能力

Joao D de Agostini Losano¹, Junxiao Ren¹, Feng Yue¹

¹Department of Animal Sciences, University of Florida, Gainesville, FL, USA.

Journal of animal science

|June 16, 2025

概括

高分辨率呼吸计揭示了一种新的精子特征,储备呼吸能力 (SCR),它与运动性一起,在人工授精计划中显著改善了公牛生育预测.

科学领域:

动物科学动物科学
生殖生物学生殖生物学
生物能源学生物能源学

背景情况:

标准的精液评估对公牛生育能力的预测价值有限.
单靠精子动力学评估无法区分高和低生育率样本.
线粒体功能可能为精子质量和生育能力提供新的见解.

研究的目的:

为了评估Oroboros Oxygraph-2k (O2k) 用于测量冷解的公牛精子中的线粒体呼吸.
评估线粒体呼吸,精子动力学和牛肉与乳制品计划中的生育能力之间的关系.
开发一个模型,以使用新的精子特征来改进公牛生育率估计.

主要方法:

分析了高和低生育率安格斯公牛的冷保存精子.
用CASA系统评估精子动力学.
线粒体呼吸被测量使用O2k系统在解后0和24小时.

主要成果:

在高生育率和低生育率组之间没有发现动力学特征的显著差异.
备用呼吸能力 (SCR) 是一种新型O2k衍生的特征,在高生育性公牛中显著更高.
结合SCR,渐进性运动,总运动和复合I呼吸的生育模型显示出高预测精度 (R2 = 0.81).

结论:

高分辨率线粒体评估识别了新的精子特征,以提高生育预测.
备用呼吸能力 (SCR) 是公牛生育能力的一个新型指标.
将线粒体呼吸数据与动力学参数结合起来,可以提高公牛生育率估计的准确性.

关键词:

人工授精是一种人工授精.生物能源生物能源学牛肉类的牛肉类的牛肉类化保存的方法线粒体中的线粒体.精子精子精子精子

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