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Updated: Sep 19, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

SCO-ph:微流动动态表型平台,用于单细胞酸化的高通量选.

Hyejoong Jeong^1,2, Emilia A Leyes Porello¹, Jean G Rosario²

¹Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Small (Weinheim an der Bergstrasse, Germany)

|June 16, 2025

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这项研究引入了一种微流体系统,用于单细胞光学pH分析 (SCO-pH),以测量细胞代谢. 高通量SCO-pH系统可以根据细胞外酸化动态区分细胞.

科学领域:

生物技术是生物技术.
细胞生物学细胞生物学
分析化学分析化学

背景情况:

量化高通量代谢测试对于单细胞表型化动态至关重要.
细胞外酸化是一种关键的代谢表型,与瘤性等细胞过程有关.

研究的目的:

开发一种多功能微流体系统,用于单细胞光学pH分析 (SCO-pH).
为了实现高通量,时间细胞外pH分析,用于动态单细胞表型化.

主要方法:

在微流体系统中,单个细胞在140-pL液滴中进行细分.
在2D阵列中固定大约4万个滴子进行分析.
封装一个细胞无透的pH探针,以光为基础的pH量化在每个滴.

主要成果:

通过监测细胞外酸化,SCO-pH系统成功地区分了经过高糖解的细胞和未经处理的细胞.
在细胞外酸化动态中观察到单细胞异质性.
该系统展示了基于代谢活动区分细胞的能力.

结论:

开发的SCO-pH系统为动态单细胞表型化提供了一种高通量方法.

关键词:

细胞外酸性化细胞外酸性化葡萄糖溶解,高通量输出.微流体学在微流体学方面现型定制现型定制一个单细胞的单细胞.

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这项技术对于需要分析细胞代谢和异质性的应用非常有价值.

SCO-pH有助于研究细胞代谢及其在各种生物环境中的影响.