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相关概念视频

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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相关实验视频

Updated: Sep 19, 2025

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
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Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

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通过定量流细胞测量来准确评估目标表达的建议.

Tamara Lekishvili1, Maxime Moulard2, Sarah Jetzer1

  • 1Molecular Partners AG, Zürich, Switzerland.

Cytometry. Part A : the journal of the International Society for Analytical Cytology
|June 16, 2025
PubMed
概括
此摘要是机器生成的。

这项研究通过确定影响抗体结合能力 (ABC) 测量的关键因素来增强定量流细胞计. 提供了强大的和可重现的抗原表达分析的建议.

关键词:
抗体结合能力的抗体结合能力.定量流动细胞计量学 定量流动细胞计量接收器密度 接收器密度标准化 标准化 标准化

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The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
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A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

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相关实验视频

Last Updated: Sep 19, 2025

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科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学
  • 生物技术是生物技术.

背景情况:

  • 流细胞计量量化细胞特征,如抗原表达.
  • 抗体结合能力 (ABC) 对于估计抗原密度和联体结合部位至关重要.
  • 报告的ABC值的变化需要测试标准化.

研究的目的:

  • 为了应对获得可重复的定量流细胞计数据的挑战.
  • 为准确的抗原表达评估提供方法建议.
  • 为了确定ABC计算中变化的来源.

主要方法:

  • 对影响ABC值的因素进行全面调查.
  • 评估仪器 (传统和全频谱),抗体,试剂,矩阵,细胞密度,自光和定量套件.
  • 实施一个系统和综合的方法.

主要成果:

  • 确定了ABC计算变化的主要来源.
  • 通过纵向研究证明了试验稳定性.
  • 建立了一个支持生物标志物评估的协议.

结论:

  • 准确的抗原表达分析需要标准化的定量流细胞计方法.
  • 方法建议提高了ABC值的可靠性和可重复性.
  • 已建立的协议有助于在各种情况下对生物标志物进行评估.