来自Arabidopsis thaliana的CCA添加酶的晶体结构
Xiao Wang1, Yuan-Yuan Li1, Zi-Yan Dou1
1School of Life Sciences, Anhui University, Hefei, Anhui 230601, China.
Journal of structural biology: X
|June 16, 2025
概括
阿拉比多普西斯·塔利亚纳 (Arabidopsis thaliana) 的晶体结构的CCA添加酶 (AtCCA) 显示出其体域中的独特特征. 这些结构上的差异可能解释了它在植物中的独特的tRNA结合和多点能力.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 植物生物化学 植物生物化学
背景情况:
- tRNA的3'-终端CCA序列对于氨基酸附着和核糖体定位至关重要.
- 植物II类CCA添加酶是核编码的,但准多个器官,包括核,细胞质,塑和线粒体.
- 植物CCA添加酶功能和向的结构基础在很大程度上是未知的.
研究的目的:
- 为了确定来自Arabidopsis thaliana (AtCCA) 的CCA添加酶的晶体结构.
- 为了研究AtCCA和其他II类CCA添加酶之间的结构差异.
- 探索AtCCA独特的tRNA结合和器官向的潜在机制.
主要方法:
- 进行X射线晶体学以确定AtCCA的3D结构.
- 与来自其他物种的同类CCA添加酶的结构比较.
- 对AtCCA-tRNA复合物的建模.
主要成果:
- 解决了AtCCA的晶体结构,揭示了与其他II类CCA添加酶的整体相似性,但在体域中存在显著差异.
- 在身体和尾部域内,确定了AtCCA独特的三个特定区域.
- 建模表明,与其同类相比,AtCCA可能表现出明显的tRNA结合模式.
结论:
- 特别是在体域中,AtCCA的独特结构特征可能有助于其特定的tRNA结合特性.
- 这些已识别的区域也可能在多个目标分类AtCCA到各种细胞区中发挥作用.
- 这项研究提供了关于植物CCA添加酶的功能和局部性的结构性见解.
更多相关视频
09:15Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
Published on: January 10, 2018
10.0K
13:35Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota
Published on: May 23, 2025
604
相关概念视频
ATP Synthase: Structure
13.2K
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
13.2K
Allosteric Proteins-ATCase
5.9K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.9K
Structure of Cadherins
3.6K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
3.6K
ATP Synthase: Mechanism
15.3K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
15.3K
C4 Pathway and CAM
46.3K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
46.3K
Protein Complex Assembly
11.1K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
11.1K
