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在Yarrowia lipolytica中对基因和蛋白质表达模式的定量多重分析

Erin Bredeweg¹, Galya Orr¹, Dehong Hu¹

¹Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, USA.

Current research in microbial sciences

|June 16, 2025

概括

这项研究揭示了Xbp1p转录因子如何控制酵母静止和营养储存. 单细胞分析表明,复杂的Xbp1p动态对于理解细胞反应至关重要.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
微生物学微生物学

背景情况:

油性酵母 (Yarrowia lipolytica) 是研究脂质积累的一个模型生物.
营养物质枯竭引发的静止,涉及从细胞分裂转向资源封存的转变.
已知转录因子Xbp1p调节了进入这种静止状态的过程.

研究的目的:

研究Xbp1p在单细胞水平调节静止和营养分离中的作用.
在耗下分析Yarrowia lipolytica*中Xbp1p的蛋白质和mRNA转录动态.
了解细胞对营养应激反应的异质性.

主要方法:

单分子光在位杂交 (smFISH) 用于转录本地化和量化.
定量聚合酶链反应 (qPCR) 用于基因表达分析.
光显微镜用于单细胞水平的蛋白质定量.

主要成果:

在单个细胞中观察到Xbp1p蛋白和mRNA水平的异质反应.
在野生类型与Δ*xbp1*突变细胞中证明了Xbp1p下游目标的差异调节.
量化了蛋白质和转录水平,揭示了耗尽期间的复杂动态.

结论:

单细胞分辨率对于准确描述酵母细胞动态和调节过程至关重要.
Xbp1p在调节过渡到静止和营养储存方面发挥着复杂的作用.
在Xbp1p动态中的细胞异质性影响了人口层面对环境线索的反应.

关键词:

光在现场混合化中的光.脂质代谢脂质代谢是一种静止是一种静止.单细胞机是一种单细胞机.单分子显微镜的显微镜.雅罗维亚 (Yarrowia lipolytica) 是一个高脂的植物.

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