舌头状细胞癌衍生外体miR-21-5p通过促进M2巨细胞两极分化影响瘤进展
Zixian Xu1,2, Xin Fang2,3, Shan Wang1
1Department of Basic Science of Stomatology, Kunming Medical University School and Hospital of Stomatology, 1168 Chunrong West Road, Chenggong Zone, Kunming, 650500, China.
Non-coding RNA research
|June 16, 2025
概括
舌状细胞癌 (TSCC) 外体通过miR-21-5p促进M2巨细胞的两极分化,有助于瘤的进展. 向外体miR-21-5p为TSCC提供了一个潜在的治疗策略.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 外体细胞介导细胞间通信,对于瘤微环境 (TME) 调节至关重要.
- 瘤衍生的外体微RNAs (miRNAs) 影响瘤相关的巨细胞 (TAM) 两极化.
- 在舌头状细胞癌 (TSCC) 中对TAMs的外体miRNA调节机制尚未完全理解.
研究的目的:
- 调查TSCC中的外体miRNAs在巨细胞极化中的作用.
- 阐明TSCC衍生的外体细胞调节TAMs的分子机制.
- 评估TSCC中M2巨细胞透的预后意义.
主要方法:
- 小RNA测序TSCC衍生的外体,以识别关键的miRNAs.
- 在体外实验中评估由TSCC外基因组诱导的巨细胞极化.
- 西方模糊分析参与M2极化中的信号通路.
- 对患者数据进行生物信息分析 (ssGSEA,考克斯回归,生存分析).
主要成果:
- miR-21-5p在TSCC衍生的外体中高度表达,并与M2 TAM两极分化相关.
- 通过抑制ERK1/2酸化,TSCC外体促进了M0巨细胞的两极化到M2表型.
- 在TSCC TME中观察到M2巨丰富,与患者预后不佳相关.
结论:
- 由TSCC衍生出的异位体miR-21-5p驱动M2巨细胞的两极分化,有助于TSCC的进展.
- 在TSCC中,M2巨细胞透是预后不良的因素.
- 向外体miR-21-5p为TSCC提供了潜在的治疗途径.
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