相关实验视频

Updated: Sep 19, 2025

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

在患有肺腺癌的患者中,TIM-1的分布特征和预后值

Junxu Wen¹, Wenhua Yun¹, Yu Chen¹

¹Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.

Frontiers in immunology

|June 16, 2025

概括

表达B细胞的T细胞免疫球蛋白和粘素域含蛋白1 (TIM-1) 抑制了肺腺癌中的免疫反应. 较低的TIM-1+B细胞密度与改善的生存率相关,表明TIM-1+B细胞作为治疗点.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
细胞生物学细胞生物学

背景情况:

T细胞免疫球蛋白和含有粘素域蛋白1 (TIM-1) 促进瘤的进展.
瘤微环境 (TME) 中TIM-1的作用和分布尚未完全理解.

研究的目的:

研究TIM-1阳性免疫细胞在肺腺癌 (LUAD) 中的分布.
确定TME和瘤排水淋巴结 (TDLN) 中TIM-1阳性免疫细胞的预后意义.

主要方法:

在126个LUAD患者组织样本上进行多重免疫光 (mIF).
在原发性瘤和TDLN中对TIM-1阳性CD8+T细胞和B细胞的分析.

主要成果:

在TDLN中,TIM-1+CD8+T细胞和TIM-1+B细胞比在原发性瘤中更为丰富.
在原发性瘤和TDLN中,较低的TIM-1+B细胞密度与较长的整体存活期 (OS) 和无病存活期 (DFS) 相相关.
在TDLN中TIM-1+B细胞密度较高与瘤中成熟的三级淋巴体结构 (TLS) 减少和CD8+T细胞密度较低有关.

结论:

在LUAD中,TIM-1+B细胞起着免疫抑制作用.
TIM-1+B细胞可以抑制TLS成熟,并降低CD8+T细胞密度,从而导致免疫抑制.
TIM-1+B细胞代表了LUAD治疗的潜在治疗点.

关键词:

一个TIM-1的TIM-1.肺部腺癌的癌症.预后预后预后三级淋巴体结构的第三级淋巴体结构瘤微环境是一个微环境.瘤排水淋巴结的淋巴结.

更多相关视频

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

Published on: August 14, 2019

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression

Published on: January 19, 2019

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...