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Updated: Sep 19, 2025

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

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催化无效的Cas9减弱了DNA末端切除:对于区域受限随机突变发生的潜在应用

Suchin Towa1, Satoshi Okada1, Takashi Ito1

  • 1Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

iScience
|June 16, 2025
PubMed
概括

研究人员开发了一种新的基因组工程方法,使用修改后的Cas9蛋白来限制DNA突变到特定区域. 这种技术控制了DNA修复过程,为未来的应用提供了精确的基因编辑.

关键词:
生物科学研究方法 生物科学研究方法模型生物模型生物.分子遗传学 分子遗传学基因学的技术在遗传学.

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