短期电刺激影响心脏细胞结构和功能
Kristen Allen1, Natalie Pachter1, Abigail Bandl1
1Department of Biomedical Engineering, Binghamton University, The State University of New York, Binghamton, New York 13902, USA.
Journal of tissue engineering and regenerative medicine
|June 16, 2025
概括
短期电刺激 (ES) 促进诱导多能干细胞衍生心肌细胞 (iPSC-CMs) 的早期成熟. 这种定制生物反应器通过改善iPSC-CM成熟和心脏纤维细胞对齐,显示出对心脏组织工程的潜力.
科学领域:
- 心血管研究研究心血管研究
- 干细胞生物学 干细胞生物学
- 生物医学工程 生物医学工程
背景情况:
- 诱导多能干细胞衍生的心肌细胞 (iPSC-CMs) 对于建模心脏发育和疾病至关重要.
- iPSC-CMs的成熟需要模仿心脏的复杂环境,通常使用电刺激 (ES).
- 之前的研究表明,基于ES参数的iPSC-CM成熟结果是可变的,这导致了最佳刺激方案的不确定性.
研究的目的:
- 开发一种低成本的定制生物反应器,用于对二维细胞培养物的可调节电刺激 (ES).
- 调查短期ES后iPSC-CMs早期成熟迹象的时间表.
- 评估生物反应器刺激心脏纤维细胞 (cFB) 和诱导细胞对齐的能力.
主要方法:
- 设计并实施了一种定制的生物反应器,用于对2D细胞单层进行受控,可调节的ES输送.
- 暴露在短期ES的iPSC-CM和cFB中.
- 分析了iPSC-CM收缩性和蛋白质表达的变化.
- 在刺激后评估cFB对齐.
主要成果:
- 与非刺激对照组相比,经过短期ES后,受刺激的iPSC-CMs表现出早期成熟的迹象.
- 收缩性和蛋白质表达的变化表明ES治疗的iPSC-CMs中的细胞重新排列和部分成熟.
- 生物反应器显示了诱导心脏纤维细胞 (cFBs) 对齐的潜力.
结论:
- 通过定制生物反应器的短期ES可以在3-4天内在iPSC-CM中诱导可测量的早期成熟.
- 生物反应器促进了将其集成到用于心脏研究的标准细胞培养平台中.
- 这项技术有望通过促进iPSC-CM成熟和细胞对齐来推进心脏组织工程.
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