Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Electrophysiology of Normal Cardiac Rhythm01:19

Electrophysiology of Normal Cardiac Rhythm

6.9K
The normal cardiac rhythm is a synchronized electrical activity that facilitates the regular and coordinated contraction of the heart muscle. This process is essential for efficient blood circulation throughout the body. The fundamental elements involved in establishing and maintaining this rhythm include the unique electrical properties of cardiac muscle cells, the sinoatrial (SA) node's pacemaker function, the specialized conducting system, and the ionic mechanisms underlying each phase...
6.9K
Cardiac Action Potential01:30

Cardiac Action Potential

2.7K
Cardiac action potentials are essential for proper heart function, enabling the rhythmic contractions needed for adequate blood circulation. Nodal cells and Purkinje fibers, specialized for electrical conduction, generate these action potentials.
The cardiac action potential process involves a series of phases characterized by the movement of ions across the cardiac cell membranes, leading to the depolarization and repolarization of the cardiac myocytes.
Ionic Basis of Cardiac Action Potentials
2.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Exogenous Extracellular Matrix in an Environmentally-Mediated In Vitro Model for Cardiac Fibrosis.

Journal of biomedical materials research. Part A·2025
Same author

Exogenous ECM in an environmentally-mediated <i>in vitro</i> model for cardiac fibrosis.

bioRxiv : the preprint server for biology·2024
Same author

Engineered Cardiac Microtissue Biomanufacturing Using Human Induced Pluripotent Stem Cell Derived Epicardial Cells.

bioRxiv : the preprint server for biology·2024
Same author

Single-Cell Analysis Identifies NOTCH3-Mediated Interactions between Stromal Cells That Promote Microenvironment Remodeling and Invasion in Lung Adenocarcinoma.

Cancer research·2024
Same journal

RETRACTION: Effect of Surface Modification of Nanofibres with Glutamic Acid Peptide on Calcium Phosphate Nucleation and Osteogenic Differentiation of Marrow Stromal Cells.

Journal of tissue engineering and regenerative medicine·2026
Same journal

Mechanical Tuning of the Cell Microenvironment Using a Biomimetic Hydrogel System for Articular Cartilage Tissue Engineering.

Journal of tissue engineering and regenerative medicine·2026
Same journal

Pirfenidone Attenuates Fibrosis and Neovascularization in 3D Spheroid-Laden Hydrogel Culture.

Journal of tissue engineering and regenerative medicine·2026
Same journal

Cranial Defect Reconstruction With Custom 3D-Printed Hydroxyapatite Scaffolds Augmented With rhBMP-2 or Dipyridamole in a Nonhuman Primate Model.

Journal of tissue engineering and regenerative medicine·2026
Same journal

Collagen-Based Scaffolds for Meniscal Repair and Regeneration.

Journal of tissue engineering and regenerative medicine·2026
Same journal

Recent Advancements in the Generation and Application of Therapeutic Cell Populations for Lung Epithelial Repair.

Journal of tissue engineering and regenerative medicine·2026
查看所有相关文章

相关实验视频

Updated: Sep 19, 2025

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential
07:41

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential

Published on: January 18, 2019

7.7K

短期电刺激影响心脏细胞结构和功能

Kristen Allen1, Natalie Pachter1, Abigail Bandl1

  • 1Department of Biomedical Engineering, Binghamton University, The State University of New York, Binghamton, New York 13902, USA.

Journal of tissue engineering and regenerative medicine
|June 16, 2025
PubMed
概括
此摘要是机器生成的。

短期电刺激 (ES) 促进诱导多能干细胞衍生心肌细胞 (iPSC-CMs) 的早期成熟. 这种定制生物反应器通过改善iPSC-CM成熟和心脏纤维细胞对齐,显示出对心脏组织工程的潜力.

关键词:
这是心肌细胞 (cardiomyocytes).收缩性 收缩性的电刺激是一种电刺激.在体外建模 in vitro 建模诱导的多能干细胞干细胞.成熟 成熟 成熟 成熟.

更多相关视频

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
10:11

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

Published on: May 6, 2017

9.2K
Bidirectional Electrical and Optoelectronic Interfaces in Healthy and Ischemic Ex Vivo Rat Hearts
08:40

Bidirectional Electrical and Optoelectronic Interfaces in Healthy and Ischemic Ex Vivo Rat Hearts

Published on: July 18, 2025

275

相关实验视频

Last Updated: Sep 19, 2025

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential
07:41

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential

Published on: January 18, 2019

7.7K
Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
10:11

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

Published on: May 6, 2017

9.2K
Bidirectional Electrical and Optoelectronic Interfaces in Healthy and Ischemic Ex Vivo Rat Hearts
08:40

Bidirectional Electrical and Optoelectronic Interfaces in Healthy and Ischemic Ex Vivo Rat Hearts

Published on: July 18, 2025

275

科学领域:

  • 心血管研究研究心血管研究
  • 干细胞生物学 干细胞生物学
  • 生物医学工程 生物医学工程

背景情况:

  • 诱导多能干细胞衍生的心肌细胞 (iPSC-CMs) 对于建模心脏发育和疾病至关重要.
  • iPSC-CMs的成熟需要模仿心脏的复杂环境,通常使用电刺激 (ES).
  • 之前的研究表明,基于ES参数的iPSC-CM成熟结果是可变的,这导致了最佳刺激方案的不确定性.

研究的目的:

  • 开发一种低成本的定制生物反应器,用于对二维细胞培养物的可调节电刺激 (ES).
  • 调查短期ES后iPSC-CMs早期成熟迹象的时间表.
  • 评估生物反应器刺激心脏纤维细胞 (cFB) 和诱导细胞对齐的能力.

主要方法:

  • 设计并实施了一种定制的生物反应器,用于对2D细胞单层进行受控,可调节的ES输送.
  • 暴露在短期ES的iPSC-CM和cFB中.
  • 分析了iPSC-CM收缩性和蛋白质表达的变化.
  • 在刺激后评估cFB对齐.

主要成果:

  • 与非刺激对照组相比,经过短期ES后,受刺激的iPSC-CMs表现出早期成熟的迹象.
  • 收缩性和蛋白质表达的变化表明ES治疗的iPSC-CMs中的细胞重新排列和部分成熟.
  • 生物反应器显示了诱导心脏纤维细胞 (cFBs) 对齐的潜力.

结论:

  • 通过定制生物反应器的短期ES可以在3-4天内在iPSC-CM中诱导可测量的早期成熟.
  • 生物反应器促进了将其集成到用于心脏研究的标准细胞培养平台中.
  • 这项技术有望通过促进iPSC-CM成熟和细胞对齐来推进心脏组织工程.