Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Letter to the editor.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2026

Same author

Impact of Chemical Aging on Venison Processing Knife Topography and Recoverable Chronic Wasting Disease Prion Seeding Activity.

Pathogens (Basel, Switzerland)·2026

Same author

Generating geochemical and mineralogy distributions of soil in the conterminous United States using Bayesian hierarchical spatial models.

MethodsX·2026

Same author

Prion seeding activity in DNA extractions: implications for laboratory biosafety.

Prion·2026

Same author

Predation vs. Parasitism: A Case Study of Indigenous Co-Stewardship and Science Co-Production to Measure Temporal Shifts in Moose Mortality on Ancestral Lands of the Grand Portage Ojibwe.

Ecology and evolution·2026

Same author

Going Mobile: Using Portable Genomic Technologies for PCR-Free In Situ Species Identification and Real-Time Molecular Systematics.

Ecology and evolution·2025

Search research articles

相关实验视频

Updated: Sep 19, 2025

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Published on: September 29, 2017

在土壤中检测子的RT-QuIC优化.

Madeline K Grunklee^1,2, Stuart S Lichtenberg^2,3, Gage Rowden^2,3

¹Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, 1365 Gortner Avenue, St. Paul, MN 55108, USA.

|June 16, 2025

概括

此摘要是机器生成的。

研究人员优化了实时震动诱导转换 (RT-QuIC) 试验,以检测明尼苏达州土壤中的慢性消耗疾病 (CWD) 子. 这种增强的测定方法可以确保在环境样本中准确检测CWD子.

关键词:

在CWD中使用CWD.鹿类动物鹿类动物鹿类动物污染污染污染污染环境环境环境环境实时震动诱导的转换试验.土壤提取提取土壤用RT-QuIC进行土壤处理以检测子.

更多相关视频

Prion Safety Laboratory Swipe Test

Prion Safety Laboratory Swipe Test

Published on: February 14, 2025

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

相关实验视频

Last Updated: Sep 19, 2025

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Published on: September 29, 2017

Prion Safety Laboratory Swipe Test

Prion Safety Laboratory Swipe Test

Published on: February 14, 2025

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

科学领域:

环境科学环境科学
神经科学是一个神经科学.
兽医医学兽医医学兽医医学

背景情况:

慢性衰竭疾病 (CWD) 是一种致命的病,影响子宫动物.
慢性疾病子 (PrPSc) 通过动物废物和尸体污染环境,持续多年.
由于复杂的土壤-子相互作用,在土壤中检测CWD子很困难.

研究的目的:

优化实时地震诱导转换 (RT-QuIC) 试验,用于在明尼苏达州土壤中检测CWD子.
开发可靠的方法来区分真正的子信号和土壤诱导的假阳性信号.
为了验证RT-QuIC测定对环境子检测的灵敏度和特异性.

主要方法:

进行了负控制实验,以完善土壤提取协议.
进行了土壤增实验,以验证RT-QuIC的性能.
利用统计评估来提高试验的特异性和灵敏性.

主要成果:

确定了最大点比 (MPR) 作为在土壤中准确检测PrPSc的关键指标.
成功优化RT-QuIC用于明尼苏达州土壤中可靠的CWD子检测.
证明了测试能够区分真正的子活动与背景噪声的能力.

结论:

优化的RT-QuIC协议提供了一种可靠的方法来检测明尼苏达州土壤中的CWD子.
最大点比 (MPR) 度量对于在复杂的环境矩阵中准确检测子至关重要.
这些优化策略适用于全球其他土壤类型的RT-QuIC验证.