通过抑制CEND1通过LSD1调节心脏再生
Huahua Liu1,2, Jinling Dong2, Shuang Liu3
1Department of Cardiology, First Affiliated Hospital; Cardiometabolic Innovation Center of Ministry of Education, Xi'an Jiaotong University, Xi'an, China.
Theranostics
|June 16, 2025
概括
激活LSD1和抑制Cend1促进小鼠在受伤后的心脏再生. 这种表观遗传轴对于修复新生儿和成人心脏损伤至关重要.
科学领域:
- 心血管生物学 心血管生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 再生医学是一种再生医学.
背景情况:
- 重新激活心肌细胞增殖是心脏再生的关键.
- LSD1-CEND1轴在表观遗传上抑制了Cend1,这对心肌细胞增殖和心脏发育至关重要.
研究的目的:
- 研究LSD1-CEND1轴在心脏受伤后的再生和修复中的作用.
- 探索改善心脏修复的治疗策略.
主要方法:
- 使用心肌细胞特异性Lsd1淘汰/过度表达和Cend1无/过度表达的小鼠模型.
- 通过切除 (新生儿) 或冠状动脉结合 (成人) 诱导心脏损伤.
- 评估心脏功能 (心声学) 和组织学 (马森染色);分析分子变化 (RNA-seq,qPCR,西斑,免疫染色).
主要成果:
- Lsd1 缺失影响新生儿心脏再生;Lsd1 过度表达改善了它.
- 作为心肌细胞循环的抑制剂,Cend1在Lsd1损失时被上调.
- 过度表达Cend1阻碍了再生;Cend1的删除促进了再生,增强了心肌细胞的增殖,新血管和巨细胞的激活.
- 由Lsd1损失引起的再生缺陷通过Cend1删除得到了挽救.
- 在成年小鼠中,Lsd1过度表达或Cend1删除改善了心肌梗塞后的心脏功能.
结论:
- 在新生儿和成年小鼠中,对CEND1的LSD1依赖性抑制对心脏再生至关重要.
- 向LSD1激活和CEND1抑制可能为内源性心脏修复提供治疗策略.
相关概念视频
01:15Neurogenesis and Regeneration of Nervous Tissue
1.1K
In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
1.1K
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
01:19Overview of Regeneration and Repair
4.3K
Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...
Regeneration
All animals have varying degrees of...
4.3K