用玉米炉连续浸泡和固态发酵的三级级流程生产乙基丁酸盐
Dongsheng Xue1, Jie Huang1, Jueyan Chen1
1National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, 430068 People's Republic of China.
3 Biotech
|June 16, 2025
概括
这项研究表明,使用级联发酵过程,从玉米中连续生产乙二甲酸盐. 综合系统有效地将纤维素转化为乙烯基丁酸盐,突出显示了一种可持续的生物工艺.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 可持续化学 可持续化学
背景情况:
- 玉米炉是一种丰富的纤维素生物质,对直接利用来说是一个挑战.
- 对生物炼油厂来说,有效地将生物质转化为增值化学物质,如乙二甲酸盐,至关重要.
研究的目的:
- 利用玉米炉开发一种用于生产乙烯基丁酸盐的连续发酵工艺.
- 研究生物反应堆级联中固定酶和微生物的协同作用.
主要方法:
- 使用了一种连锁系统,集成了一个气体升降生物反应器,无氧发酵器和无氧固态发酵器.
- 动不动的黑色阿斯伯菌 (细胞酶) 和大脑菌 (雌激酶) 用于现场水解和化.
- 玉米炉被用作连续发酵的基质.
主要成果:
- 连续生产乙基丁酸盐的度从40.0小时到120.0小时达到30.55g/kg.
- 细胞酶和雌激酶活动分别记录为9.16 U/mL和29.16 U/mL.
- 玉米火纤维素的水解比率为55.4%,酸盐与乙酸盐的比率为6.72.2.
结论:
- 级联生物反应器系统有效地将玉米转化为乙基丁酸盐,无需外源酶.
- 该过程在现场证明了糖化和化,在微生物之间共享代谢负担.
- 这种综合方法为生物化学品生产提供了一种可持续和高效的方法.
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