相关实验视频
Updated: Sep 19, 2025
13:04
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Published on: March 1, 2019
9.0K
替代转录增加了长非编码RNA中的异形复杂性,并改变了它们在癌症中的功能
Max Bone1,2, Gareth J Inman1,2
1School of Cancer Sciences, University of Glasgow, United Kingdom.
Non-coding RNA research
|June 16, 2025
概括
转录起点和终点位点的变化显著影响长非编码RNA (lncRNA) 多样性,而不是替代拼接. 了解这种变异对于lncRNA功能和精确的癌症疗法至关重要.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 癌症研究 癌症研究
背景情况:
- 替代拼接得到了很好的研究,但转录起点和终点位点的变异是RNA同位素多样性的主要驱动因素.
- 长非编码RNAs (lncRNAs) 表现出显著的异形多样性,受转录开始和结束地点变化的影响.
- 异常的 lncRNA 异型在癌症发病和进展中起作用.
研究的目的:
- 审查转录开始和结束变异在lncRNA生物学中的低估的作用.
- 突出转录变异对lncRNA结构,功能和多样性的影响.
- 强调了解ncRNA转录变异对于癌症研究和治疗的重要性.
主要方法:
- 文献综述侧重于 lncRNAs 中的转录起点和终点位点变异.
- 分析单核酸变化如何影响 lncRNA 的特性.
- 对癌症生物学和反感性寡核酸 (ASO) 疗法的影响的讨论.
主要成果:
- 转录起点和终点位点的变化比替代拼接更为促进 lncRNA 异形多样性.
- 转录开始/结束部位的变化可以显著改变 lncRNA 的结构,稳定性和功能.
- 这些变异与癌症发展有关,并提供潜在的治疗点.
结论:
- 转录起点和终点位点的变异是 lncRNA 多样性的关键,但尚未研究的决定因素.
- 了解ncRNA转录变异对于开发向癌症疗法至关重要.
- 利用 lncRNA 转录变异可能导致新的精确治疗策略.
相关概念视频
01:32RNA Splicing
57.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
57.2K
02:39lncRNA - Long Non-coding RNAs
9.0K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
02:18Alternative RNA Splicing
21.8K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.8K
01:20Types of RNA
6.6K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
6.6K
01:36What is Gene Expression?
9.2K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
9.2K
03:18Non-LTR Retrotransposons
11.9K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.9K