通过触线线装置制造对齐的聚基酸纤维支架
Md Mazbah Uddin1, Ummay Mowshome Jahan2, Vijay Mohakar1
1Department of Textiles, Merchandising, and Interiors, University of Georgia, 305 Sanford Dr., Athens, Georgia 30602, United States.
ACS omega
|June 16, 2025
概括
触摸织制造的聚3-基酸 (PHB) 纤维用于组织工程. 用原基改造的支架增强了细胞活力和增殖,显示出作为电旋转可扩展的替代方案的前景.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 聚合物科学 聚合物科学
背景情况:
- 聚3-基酸盐 (PHB) 是一种可生物降解的聚合物,具有组织工程的潜力.
- 传统的制造方法,如电,面临着可扩展性的挑战.
- 开发用于PHB脚手架生产的替代性,可扩展的方法至关重要.
研究的目的:
- 使用触摸机制造聚3-基酸 (PHB) 纤维.
- 为控制纤维形态和脚手架对齐优化线参数.
- 评估PHB支架的生物活性和细胞相容性,特别是原功能化后,用于组织工程应用.
主要方法:
- 使用触摸来制造PHB纤维,优化了溶液度,旋转速度和料速度等参数.
- 场辐射扫描电子显微镜 (FE-SEM) 用于形态分析和纤维直径测量.
- 热重力测量分析 (TGA) 和差分扫描热量测量 (DSC) 评估了热性能和结晶性.
- 细胞活力和增殖试验 (alamarBlue,Live/Dead) 和免疫光显微镜评估了细胞对原功能化的支架的反应.
主要成果:
- 成功制造了线性PHB纤维支架,纤维直径在0.831-1.273μm之间.
- 与颗粒相比,PHB纤维具有良好的热稳定性 (开始降解~290°C) 和增加晶度 (42.5%).
- 原功能化的支架在5天内显著增强纤维细胞 (NIH3T3) 代谢活动,活力和增殖.
- 细胞显示在纤维轴上对齐,表明了脚手架引导的方向.
结论:
- 触摸是一种可行和可扩展的方法,用于生产PHB纤维支架用于组织工程.
- 原蛋白功能化显著提高PHB支架的生物活性.
- 这些PHB支架显示出促进细胞粘附,增殖和引导组织再生的潜力.
相关概念视频
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Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
01:14Generation of Straight or Branched Actin Filaments
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Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
00:57Formation of Intermediate Filaments
Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been reported.
02:50Spindle Assembly
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...