在与人类诱导的多能干细胞分化时,SIX1表达的压力诱导增强在preplacodal ectoderm的分化过程中
Seungtae Kim1, Ayumi Horikawa2, Takayoshi Yamamoto2
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
The International journal of developmental biology
|June 16, 2025
概括
机械力对于前皮外皮 (PPE) 的分化至关重要. 在特定的发育窗口中应用拉伸刺激显著增强PPE标记物的表达,表明机械影响的关键时期.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 机械生物学 机械生物学
背景情况:
- 在体内形态发生表明机械线索调节细胞分化.
- 以前的研究表明,机械力增强了人类多能干细胞 (hPSCs) 中的神经板边界 (NPB) 规范.
- 机械力在从NPB衍生而来的前皮外皮 (PPE) 规范中的作用仍然基本上是未知的.
研究的目的:
- 为了研究机械力的作用,在preplacodal外皮 (PPE) 的差异化.
- 用hPSCs来确定机械刺激是否影响PPE规格.
- 在个人防护设备开发过程中,确定对机械力敏感的关键时期.
主要方法:
- 利用拉伸室对hPSCs施加重复的拉伸刺激.
- 使用经过验证的方法诱导PPE.
- 通过定量方法分析了个人防护设备标记SIX1的表达.
- 进行免疫光染色以评估活性β-catenin水平.
主要成果:
- 从第2天到第10天或第2天到第7天应用的重复拉伸刺激显著增加了SIX1表达.
- 当从第5天到第10天应用拉伸刺激时,没有观察到SIX1表达的增加.
- 与对照组相比,拉伸样本显示活性β-catenin信号较低,表明Wnt信号减少.
- 鉴定了个人防护设备差异化过程中机械力敏感性的关键时期.
结论:
- 机械力在调节前甲外皮 (PPE) 差异化方面发挥着重要作用.
- 机械刺激的特定时间对于有效的PPE规范至关重要.
- 机械力量可能会在PPE开发过程中负面调节Wnt信号.
相关概念视频
01:06Induced Pluripotent Stem Cells
4.4K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.4K
01:32Chromatin Modification in iPS Cells
1.9K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.9K
01:29Somatic to iPS Cell Reprogramming
2.3K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K