棕色的阿尔多激素产生腺瘤邻近的脂肪组织参与阿尔多激素合成法规
Ling Xie1, Shumin Yang1, Miaoyun Chen1
1Department of Endocrinology, Sichuan-Chongqing Joint Key Laboratory of Metabolic Vascular Diseases, Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
概括
产生阿尔多的腺瘤 (APA) 改变了相邻的脂肪组织,诱导了色表型. 网酸和乳酸可能会调解这种相互作用,影响瘤的进展.
科学领域:
- 内分泌学 在内分泌学.
- 脂肪组织生物学 脂肪组织生物学
- 瘤微环境研究 研究
背景情况:
- 产生阿尔多的腺瘤 (APA) 导致过多的阿尔多分泌.
- 与APA相邻的脂肪组织与瘤进展有关.
研究的目的:
- 为了研究APA和相邻的脂肪细胞之间的相互作用.
- 为了确定APA与脂肪组织关系中的潜在调解者.
主要方法:
- 针对UCP1表达的免疫组织化学和免疫光学.
- 对UCP1和PGC1α进行mRNA分析.
- 非向性脂管学. 非向性脂管学.
- 测量视网膜酸 (RA) 和乳酸水平.
- 使用H295R细胞进行体外研究,以评估CYP11B2表达和阿尔多素分泌.
主要成果:
- 与APA相邻的脂肪组织显示出棕色化表型,脂肪细胞较小,UCP1表达增加.
- 上调的UCP1,PGC1α mRNA和改变的脂质组学证实了脂肪组织的色.
- 在APA中观察到ALDH1A2表达和视网酸 (RA) 的升高,促进脂肪细胞色.
- 邻近脂肪组织中乳酸的增加增强了H295R细胞中阿尔多素合成酶 (CYP11B2) 表达和阿尔多素分泌.
结论:
- APA与相邻的脂肪组织相互作用,诱导棕色化表型.
- 视酸 (RA) 和乳酸成为这种相互作用的潜在媒介.
- 这些发现提供了关于脂肪组织在APA微环境中的作用的见解.
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