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通过DNA触发aptamer中和酶的激活,以in situ形成可注射水凝
Connie Wen1, Yixun Wang1, Kyungsene Lee1
1Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, USA. yxw30@psu.edu.
Nanoscale horizons
|June 16, 2025
概括
一种新的可注射水凝系统使用双特异的aptamer中和酶并触发DNA. 这个系统在与血液接触时迅速形成水凝,使精确的栓塞和出血控制成为可能.
科学领域:
- 生物材料科学 生物材料科学
- 生物医学工程 生物医学工程
- 生物化学 生化学
背景情况:
- 可以注射的水凝对于血管形和出血控制至关重要.
- 理想的水凝在与血液接触时形成,以控制可注射性.
- 目前用于快速,现场水凝形成的系统是有限的.
研究的目的:
- 开发一种新的可注射水凝系统,用于快速的in-situ凝.
- 为了利用双特异的阿帕特默中和酶并触发DNA机制.
- 为了证明该系统在栓塞应用中的有效性.
主要方法:
- 使用双特异性阿帕特马中和酶制备一种预凝溶液.
- 引入触发DNA以启动水凝的形成.
- 在体外测试动脉瘤和内血管栓塞模型.
主要成果:
- 该系统在化前状态下与体保持稳定.
- 与触发DNA的接触迅速诱导了水凝的形成.
- 试管体内栓塞模型显示,在DNA给药后,水凝的形成加速.
结论:
- 成功开发了一种基于阿普坦-酶相互作用的新型可注射水凝系统.
- 该系统展示了由特定DNA序列触发的快速和可控制的水凝形成.
- 这项技术对先进的栓塞和出血控制疗法具有前景.
相关概念视频
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...