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Updated: Sep 19, 2025

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Dissection of Larval Zebrafish Gonadal Tissue
Published on: April 26, 2017
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在普通话鱼 (Siniperca chuatsi) 的性别分化过程中,生殖腺发育的早期时间动力学和性变异基因表达
Yi Rong1,2, Yuanyuan Wang1,2, Jinzhi Zhao1,2
1State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Hubei Hongshan Laboratory, Wuhan, 430072, China.
Marine biotechnology (New York, N.Y.)
|June 16, 2025
概括
这项研究揭示了鱼性发育中的关键分子事件. 性别确定发生在受精后27天之前,分化在雌性中完成51天,在雄性中完成57天.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 鱼 (Siniperca chuatsi) 是一个重要的淡水水产物种.
- 鱼的早期性腺发育和性别分化的机制尚不清楚.
研究的目的:
- 研究鱼早期性腺发育和性别分化的细胞和分子过程.
- 在性发育过程中识别关键基因及其表达模式.
主要方法:
- 发生性腺发育的组织学分析 (H&E染色).
- 胚胎细胞形态和分布的免疫光分析.
- 对与性别相关的基因表达的定量分析 (cyp19a,dmrt1, amh, sox9, foxl2a, gsdf, dmrt2, dmrt3, dmc1, sycp3).
主要成果:
- 在受精后的27天和33天分别出现了oogonia和spermatogonia (dpf).
- 性别分化完成了51个dpf (女性) 和57个dpf (男性).
- 关键基因的差异表达 (例如,amh,dmrt1,cyp19a,foxl2a) 与性别发展的特定阶段相关.
结论:
- 在普通话鱼中,性别的确定发生在27dpf之前.
- 女性的性别分化发生在27至51dpf之间,而男性的性别分化发生在33至57dpf之间.
- 胚胎细胞形态学和与性别相关的基因表达提供了对鱼性发育的洞察.
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