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Updated: Sep 8, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

通过双重准策略克服癌症检测中的表皮层-介质细胞过渡挑战

Xiao-Ning Tong^1,2,3, Heng Liu^1,4, Yi He^1,4

¹The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.

|June 16, 2025

概括

研究人员开发了一种两种特异性吸收体探针 (BAptP),该探针针对EpCAM和CD71,改善癌细胞识别,并使精确的瘤成像和治疗成为可能,即使EpCAM水平有所不同.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

表皮细胞粘附分子 (EpCAM) 是关键的癌症生物标志物,但它们在表皮细胞-介质细胞过渡 (EMT) 和癌症类型变异性期间的下调限制了当前的向策略.
由于EMT期间的抗原表达变异性和损失,现有的EpCAM准技术面临挑战.

研究的目的:

设计一种新的分子癌症识别探头 (BAptP),通过同时识别EpCAM和CD71.1,增强瘤向.
克服EpCAM下调和癌症诊断和治疗变化的局限性.

主要方法:

开发两特异性体 (BAptP) 的开发,设计以结合EpCAM和CD71.
在体外验证BAptP结合特异性和在生理环境中与各种癌细胞复合形成.
在体内光成像以评估BAptP的瘤积累和保留,而不是与单性体相比.

主要成果:

在低度下,BAptP表现为稳定地与多种癌细胞结合,并形成有效的向联体受体复合体.
试验室研究证实了在复杂的生物环境中对不同类型的瘤细胞的特定识别.
在体内成像显示优异的瘤积累和长时间保留双特异的BAptP比单价的aptamers.

结论:

工程双特异性吸收体探针 (BAptP) 提供了一个强大的解决方案,用于识别癌细胞,尽管EpCAM表达的变化.
BAptP显示出作为临床研究的分子诊断工具的巨大潜力.
增强的瘤向和BAptP的保留突出了其对精确癌症治疗的承诺.

关键词:

两种两种特异性吸收体的吸收体.癌细胞检测癌细胞检测瘤成像图像检查可以瘤向向向的目标

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