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Updated: Sep 19, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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自然RNA纳米的冷EM结构

Xiaobin Ling1,2, Dmitrij Golovenko3, Jianhua Gan4

  • 1RNA Therapeutics Institute, UMass Chan Medical School, Worcester, MA, USA. xiaobin.ling@umassmed.edu.

Nature
|June 16, 2025
PubMed
概括

研究人员通过细菌和菌体的长非编码RNAs (lncRNAs) 形成的新型RNA纳米. 这些ROOLRNA结构可以被设计为研究和治疗中的潜在运输工具.

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Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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