在电发酵链延长中的多个电子捐赠者之间的协同作用:加速的定向电子转移和增强的微生物功能
Dengfei Li1, Aijuan Zhou2, Jun Mei3
1Department of Water Supply and Drainage, Taiyuan University of Technology, Taiyuan 030024, China; Shanxi-Zheda Institute of Advanced Material and Chemical Engineering, Taiyuan 030000, China.
Bioresource technology
|June 16, 2025
概括
这项研究表明,如何使用乙醇和乳酸作为电子捐赠体,可以促进电气发酵中的中链脂肪酸 (MCFA) 生产. 这种方法增强了酸盐的合成,并优化了微生物群落的废物回收利用.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 环境微生物学 环境微生物学
- 可持续化学 可持续化学
背景情况:
- 电发酵辅助链延长 (EF_CE) 将有机废物转化为有价值的中链脂肪酸 (MCFA).
- 了解多个电子捐赠者 (ED) 的监管机制对于优化 EF_CE 过程至关重要.
- 联合养EDs对微生物联盟和代谢途径的协同效应仍未得到充分研究.
研究的目的:
- 调查乙醇和乳酸作为EDs对EF_CE系统中MCFA生物合成的协同作用.
- 在多种ED条件下阐明微生物群落的转变和代谢途径规则.
- 通过优化EF_CE.提升从有机废物中提升鱼产量.
主要方法:
- 系统地探索EF_CE与联合养的乙醇和乳酸盐.
- 超基因组分析以评估微生物社区结构和基因丰富度.
- 量化MCFA产量,特别是羊.
主要成果:
- 同时食乙醇和乳酸盐显著增加了2.9-3.9倍的酸盐合成.
- 多个EDs促进了跨的微生物协会,并有利于鱼合成细菌.
- 甲基因组数据表明了关键代谢基因的转变,包括Mut的下调 (烯酸盐通路) 和ascB的上调 (木-Ljungdahl通路) 以及乙醇/乳酸盐氧化基因 (ADH,kor,cdhA).
结论:
- 作为EDs,乙醇和乳酸之间的协同作用在指导MCFA生产和重塑微生物网络方面发挥着双重作用.
- 这些发现为使用EF_CE技术提高有机废物和废水回收效率提供了宝贵的见解.
- 优化ED策略可以提高生物转化过程的经济可行性和可持续性.
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