相关实验视频

Updated: Sep 19, 2025

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

多组织单核RNA-seq揭示了猪中替代多基化细胞类型特定的调节模式

Qiuhan Wen^1,2, Zhen Wang¹, Qi Bao¹

¹Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi-omics of MARA, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.

Genome research

|June 16, 2025

概括

这项研究在261种猪细胞类型中绘制了替代多化 (APA) 地图,揭示了细胞特异的3'未翻译区域 (3' UTR) 长度模式,对基因调节和特征至关重要.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
后转录法规后转录法规后转录法规

背景情况:

替代多化 (APA) 是一个关键的转录后机制,影响基因调节和表型多样性.
对APA的单细胞分析是有限的,特别是在农业动物中,这阻碍了对细胞特异性调节作用的详细理解.

研究的目的:

在单细胞水平上构建一个全面的猪组织APA地图.
研究细胞类型特定的APA模式及其与基因表达的相关性.
确定3' UTR长度变异在特定发育过程和复杂特征中的作用.

主要方法:

来自19个猪组织的单核RNA测序 (snRNA-seq) 数据.
对261种不同的细胞类型进行分析,以确定APA事件.
3' UTR 长度与基因表达水平之间的相关性分析.

主要成果:

一个详细的APA图谱揭示了猪的组织和细胞类型特定的APA模式.
在细胞类型中平均3' UTR长度和基因表达之间存在普遍的负相关性.
特定基因 (例如CD47,GPD1) 的鉴定,具有可变的3' UTR长度,影响精子发生和肌发生.
在跨物种的神经细胞中发现了保存较长的3' UTR.
在CD47 3' UTR中特定的SNP (rs323354626) 与生殖表型的关联.

结论:

单细胞APA分析为猪提供了一层新的转录后调节数据.
细胞类型特定的3' UTR长度是细胞承诺和复杂特征的重要调节者.
APA变异有助于理解农场动物的表型多样性和遗传关联.

更多相关视频

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

相关概念视频

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...