在三阴性乳腺癌中对异质免疫微环境的多组学分析暗示UQCRFS1增强瘤进展

相关实验视频

Updated: Sep 19, 2025

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

Yuhui Tang¹, Aiqi Xu², Zhongbiao Xu³

¹State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-Sen University Cancer Center, Guangzhou, 510060, People's Republic of China. tangyh1@sysucc.org.cn.

Experimental hematology & oncology

|June 16, 2025

概括

三阴性乳腺癌 (TNBC) 具有攻击性,但其瘤免疫微环境 (TME) 异质性是关键. 这项研究确定IL32高的Tregs,恶性细胞指数 (MCI) 模型和UQCRFS1对于TNBC进展和免疫治疗反应至关重要.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
基因组学就是基因组学.

背景情况:

三阴性乳腺癌 (TNBC) 呈现出侵略性特征,导致患者预后不佳和转移增加.
瘤免疫微环境 (TME) 显著影响TNBC的进展和对免疫疗法的反应,但其异质性需要进一步调查.

研究的目的:

阐明TME在三阴性乳腺癌中的复杂异质性.
在TNBC TME中识别关键的细胞参与者和分子标记物,这些标记物会影响患者的治疗结果和治疗反应.

主要方法:

在9名未接受过治疗的TNBC患者的单细胞RNA测序,分析TME细胞类型和相互作用.
基于五个关键基因的LASSO-Cox回归的恶性细胞指数 (MCI) 模型的开发和验证.
通过空间转录组学,蛋白组学,qRT-PCR和西部涂抹,以及体外和体外功能实验来评估UQCRFS1的表达.

主要成果:

多种TME细胞子集群的表征,具有不同的细胞相互作用和功能途径.
识别与瘤逃避和较差的TNBC预后相关的高IL32调节性T细胞 (Tregs).
验证MCI模型用于预测TNBC的劣质预后和免疫疗法耐药性;UQCRFS1敲击抑制了TNBC细胞的增殖,迁移和瘤生长.

结论:

这项研究为TNBC TME异质性提供了新的见解,有助于理解TNBC生物学.
临床建议包括评估IL32高的Treg透,MCI得分和UQCRFS1表达,以改善TNBC患者的预后和治疗策略.

关键词:

在IL32高Treg中使用.免疫疗法耐药性免疫疗法耐药性多个omics的多个omics.三阴性乳腺癌是三阴性乳腺癌.瘤的免疫微环境在 UQCRFS1 中,UQCRFS1 是

更多相关视频

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

相关概念视频

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...