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在Bifidobacterium longum亚种中的Indole衍生物生物合成. 婴儿和托芬基质的可用性

Shi-Min Zhang¹, Hui-Chu Wu², Jia-He Hung³

¹Program in Molecular Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.

Microbial biotechnology

|June 17, 2025

概括

双细菌长子物种双菌长子物种 infantis从酸中生产酸-3-乙酸 (IAA) 和酸-3-碳酸 (I3CA) 等酸衍生物. 托的可用性调节了这种生产,影响了肠道健康和免疫调节.

科学领域:

微生物学微生物学
肠道微生物组研究研究
代谢工程是代谢工程.

背景情况:

双细菌长子物种双菌长子物种婴儿是一种关键的肠道殖民者.
它的新陈代谢过程,特别是从托中产生醇衍生物,对于肠道健康至关重要.
印度衍生物,如印度-3-乳酸盐 (ILA),印度-3-酸盐 (IAA) 和印度-3-碳酸 (I3CA) 在免疫调节中发挥作用.

研究的目的:

为了研究B. infantisATCC 15697生物合成ILA,IAA和I3CA的能力.
为了确定基质 () 可用性对这些代谢途径的调节作用.
使用基因组和代谢数据,为IAA和I3CA提出潜在的生物合成途径.

主要方法:

使用HPLC-PDA和LC-MS分析来识别和量化英衍生物.
B. infantis ATCC 15697是在代谢活跃,不生长的条件下培养的.
基因组分析与代谢分析进行了整合.

主要成果:

首次确认了B. infantis ATCC 15697公司的IAA和I3CA的生产.
证明了托可用性和英多尔衍生物生产之间的剂量依赖关系.
建议的IAA和I3CA的潜在生物合成途径.

结论:

B. infantis ATCC 15697生产关键的醇衍生物,其生产水平受托可用性的影响.
这些发现增强了对B. infantis在肠道健康和免疫功能中的作用的理解.
B. infantis菌株通过全细胞生物转化显示出作为生物催化剂的潜力,可以通过全细胞生物转化生产有益的醇衍生物.

关键词:

双细菌长子物种双菌长子物种婴幼儿的孩子们印度尔衍生品的印度尔衍生品印醇-3-乙酸盐的使用方法印-3-碳酸甲基的使用.印-3-乳酸盐的存在基质可用性基质可用性托芬三聚氨酸是什么

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