FLL2通过多基和CPSF73相互作用来调节Arabidopsis的发育和耐压能力
Saiqi Hao1,2, Danhui Zhao1, Congting Ye1
1Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, College of the Environment and Ecology, Xiamen University, Xiamen 361102, Fujian, China.
iScience
|June 17, 2025
概括
FLL2蛋白通过影响替代多基化 (APA) 来影响植物发育和应激反应. 用AN3661抑制CPSF73显示FLL2和CPSF73通过APA共同调节生长和适应.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因规则 基因规则
- 生物化学 生物化学
背景情况:
- 卷轴-卷轴蛋白FLL2在多基化复合体内涉及液体-液体相分离.
- FLL2在多化过程中的确切作用及其更广泛的生物功能在很大程度上仍未被描述.
研究的目的:
- 为了研究FLL2在Arabidopsis中的功能,特别是其在多基化中的作用以及与CPSF73.3的关系.
- 阐明FLL2如何影响植物发育,耐压力和基因表达调节.
主要方法:
- 利用了Arabidopsis FLL2基因中的一个发育突变SOF78.
- 使用已知抑制CPSF73的佐xaborol化合物AN3661,来探测FLL2-CPSF73的相互作用.
- 应用多A标签测序 (PAT-seq) 来分析跨转录组的替代多基化 (APA) 事件.
主要成果:
- sof78突变体表现出发育变化和改变的氧化应激耐受性.
- 用AN3661治疗模仿了一些突变表型,这表明CPSF73和FLL2.2的共同调节.
- 在AN3661治疗或氧化应激下,PAT-seq揭示了在sof78突变体中APA事件的显著变化,具有改变的多元A信号组成和规范/非规范位置使用的转变.
- 受影响的基因在与非生物压力,激素信号 (auxin,乙烯) 和透压力相关的途径中得到丰富.
结论:
- FLL2和CPSF73通过调节替代多化来共同调节植物的生长,发育和适应.
- 由FLL2和CPSF73调解的APA在植物对环境刺激和发育过程的反应中起着至关重要的作用.
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