通过AMPK/mTOR信号传递,ORM1调解了Ln-IgG诱导的足细胞损伤和自
Jie Chen1, Libin Zou2, Lu Liu3
1Department of Nephrology, Wuhan Third Hospital, Wuhan, Hubei Province, China.
Organogenesis
|June 17, 2025
概括
在狼性炎 (LN) 中,ORM1的上调会加剧细胞损伤. 减少ORM1通过AMPK/mTOR通路调节自细胞来保护细胞,为LN.提供了潜在的治疗策略.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 足细胞损伤是狼性炎 (LN) 发病的一个关键因素.
- 识别治疗点来保护细胞对于改善LN治疗结果至关重要.
- 欧罗苏木科伊德1 (ORM1) 已经成为一种潜在的候选基因,涉及到LN.
研究的目的:
- 在狼性炎模型中研究ORM1在细胞损伤中的作用.
- 探索ORM1敲击对细胞活力,亡和自的影响.
- 阐明AMPK/mTOR信号通路在ORM1介导的细胞损伤中的参与.
主要方法:
- 建立了一种狼性炎 (LN) 模型,使用与患者衍生的免疫球蛋白G (IgG) 刺激的小鼠细胞.
- 执行ORM1敲击并评估细胞活力 (CCK-8测定) 和亡 (流细胞计).
- 通过西部涂抹/免疫光分析了自标志物 (LC3II/I,p62) 并评估了AMPK/mTOR通路的激活.
主要成果:
- 在LN模型中,ORM1表达被上调.
- 降低ORM1减轻了IgG诱导的细胞损伤,减少了亡并使生命力正常化.
- 降低ORM1敲击降低了自水平的升高和调节的AMPK/mTOR信号传递 (增加了p-AMPK,降低了p-mTOR).
结论:
- 在狼性炎模型中,ORM1在细胞损伤中起着重要作用.
- 通过AMPK/mTOR信号通路,ORM1会影响细胞自.
- 准ORM1可能是治疗狼性炎的新方法.
相关概念视频
PI3K/mTOR/AKT Signaling Pathway
4.0K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
4.0K
mTOR Signaling and Cancer Progression
3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.9K
Autophagy
4.6K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.6K
The JAK-STAT Signaling Pathway
9.3K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
9.3K
Receptor Downregulation in MVBs
2.2K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.2K
TGF - β Signaling Pathway
7.7K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.7K


